BST2 Antibody (4F6) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-80604
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Cell Surface), CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 4F6
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length recombinant human BST2 protein [NCBI NP_004326].
Localization
Cell membrane, trans-Golgi network, late endosome and cytoplasm
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for BST2 Antibody (4F6) - Azide and BSA Free
![Western Blot: BST2 Antibody (4F6)Azide and BSA Free [NBP2-80604]](https://resources.rndsystems.com/images/products/BST2-Antibody-4F6-Azide-and-BSA-Free-Western-Blot-NBP2-80604-img0001.jpg "Western Blot: BST2 Antibody (4F6)Azide and BSA Free [NBP2-80604]")
Western Blot: BST2 Antibody (4F6)Azide and BSA Free [NBP2-80604]
Western Blot: BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604] - WB analysis of BST2 protein in (A) human heart lysate (B) human ovary lysate and on (C) partial recombinant BST2 protein with monoclonal BST2 antibody (clone 4F6) at a concentration of 1 ug/mL. In the tested lysates, two bands were observed which represents the glycosylated (lower intense bands) and the ubiquitinated (higher weak bands) forms of BST2 protein. Image from the standard format of this antibody.![Immunohistochemistry: BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604]](https://resources.rndsystems.com/images/products/BST2-Antibody-4F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80604-img0003.jpg "Immunohistochemistry: BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604]")
Immunohistochemistry: BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604]
Immunohistochemistry: BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604] - Analysis of BST2 protein in a section of human lung cancer (squamous cell carcinoma) using 5 ug/mL concentration of BST2 antibody (clone 4F6). The carcinoma cells depicted strong membrane-cytoplasmic BST2 positivity with more intense staining in the cellu![Flow (Cell Surface): BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604]](https://resources.rndsystems.com/images/products/BST2-Antibody-4F6-Azide-and-BSA-Free-Flow-Cell-Surface-NBP2-80604-img0004.jpg "Flow (Cell Surface): BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604]")
Flow (Cell Surface): BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604]
Flow (Cell Surface): BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604] - Flow analysis of Molt4 cells using the BST2 antibody [4F6]. The shaded purple region represents cells alone, green represents the isotype control and pink represents the BST2 antibody. Image from the standard format of this antibody.![Immunohistochemistry: BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604]](https://resources.rndsystems.com/images/products/BST2-Antibody-4F6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80604-img0002.jpg "Immunohistochemistry: BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604]")
Immunohistochemistry: BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604]
Immunohistochemistry: BST2 Antibody (4F6) - Azide and BSA Free [NBP2-80604] - Analysis of BST2 protein in a section of human endometrial carcinoma using 5 ug/mL concentration of BST2 antibody (clone 4F6). The carcinoma cells depicted distinct membrane-cytoplasmic BST2 positivity with more intense staining in the cellular membranes.Applications for BST2 Antibody (4F6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 ug/ml
Immunohistochemistry-Paraffin
5 ug/ml
Western Blot
1.0 ug/ml
Application Notes
The unprocessed form of BST2 protein is 180 amino acids long (161 amino acids in the cleaved BST) and its cleaved form undergoes further modifications namely disulfide bond formation, GPI-anchor/GPI-like-anchor binding, glycosylation, isopeptide bond formation, lipidation and Ubl conjugation. The predicted molecular weight of unmodified BST2 protein is ~17-18 kDa, whereas the post-translationally modified protein has been documented to run at ~25-36 kDa (glycosylated), 50-55 kDa (ubiquitinated) and 65-70 kDa (dimers). BST2 protein localizes mainly to the cell membranes from where it shuttles to the trans-Golgi network, late endosome and the cytoplasm. In immuno-staining assays, BST2 may develop a membrane-cytoplasmic staining pattern. This antibody is CyTOF ready.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BST-2/Tetherin
Long Name
Bone Marrow Stromal Cell Antigen 2
Alternate Names
BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin
Gene Symbol
BST2
Additional BST-2/Tetherin Products
Product Documents for BST2 Antibody (4F6) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BST2 Antibody (4F6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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