BTN3A1 Antibody (DM94)

Novus Biologicals | Catalog # NBP3-48285

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # DM94
View all CD277/BTN3A1 Primary Antibodies »

Product Specifications

Immunogen

BTN3A1 recombinant protein expressed in mammalian cells (Accession# O00481). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for BTN3A1 Antibody (DM94)

BTN3A1 Antibody (DM94)

Western Blot: Rabbit BTN3A1 mAb (DM94) [NBP3-48285]

Anti-BTN3A1 antibody at 1/1000 dilution

Lane : RPMI8226, whole cell lysate
Secondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution

Predicted band size: 33 kDa
Observed band size: 35，54，68 kDa
BTN3A1 Antibody (DM94)

ELISA: BTN3A1 Antibody (DM94) [NBP3-48285]

ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human BTN3A1 protein, mFc-His tagged protein PME100056 can bind Rabbit anti-BTN3A1 monoclonal antibody ( clone: DM94) in a linear range of 0.64-80 ng/ml.
BTN3A1 Antibody (DM94)

Western Blot: BTN3A1 Antibody (DM94) [NBP3-48285]

Anti-BTN3A1 antibody (Catalog Number# NBP3-48285) at 1/1000 dilutionLane : RPMI8226, whole cell lysateSecondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilutionPredicted band size: 33 kDaObserved band size: 35, 54, 68 kDa
BTN3A1 Antibody (DM94)

Flow Cytometry: BTN3A1 Antibody (DM94) [NBP3-48285]

Flow cytometry analysis of antigen binding of rabbit anti-human BTN3A1 mAb(NBP3-48285). (A) NBP3-48285 does not bind to MCF-7 cells that do not express BTN3A1. (B) A clear peak shift of NBP3-48285 was seen compared to the control when incubated with BTN3A1-expressing 8226 cells, indicating strong binding of NBP3-48285 to BTN3A1. Antibodies were incubated at 2 ug/mL.

Applications for BTN3A1 Antibody (DM94)

Application
Recommended Usage

ELISA

1:5000-10000

Flow Cytometry

1:100

Western Blot

1:1000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD277/BTN3A1

The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A1) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010]

Alternate Names

BT3.1, BTF5, BTN3A1, CD277

Gene Symbol

BTN3A1

Additional CD277/BTN3A1 Products

Product Documents for BTN3A1 Antibody (DM94)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BTN3A1 Antibody (DM94)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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