C. botulinum BoNT-D Light Chain Antibody [Biotin]

R&D Systems | Catalog # BAF6037

R&D Systems

Key Product Details

Species Reactivity

C. botulinum

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG
View all BoNT-D Light Chain Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant Clostridium BoNT-D Light Chain
Thr2-Ser428
Accession # P19321

Specificity

Detects C. botulinum BoNT-D Light Chain in Western blots. In Western blots, less than 10% cross-reactivity with recombinant Light Chains of BoNT-A, -B, -C1, -E, -F, and -G is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for C. botulinum BoNT-D Light Chain Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Botulinum Neurotoxin Type D Light Chain (Catalog # 6037-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BoNT-D Light Chain

BoNT-D LC (Botulinum neurotoxin serotype D light chain) is a 50-55 kDa member of the peptidase M27 family of molecules. It is the product of Clostridium botulinum, and inhibits acetylcholine release from neuromuscular junctions. This is accomplished by toxin internalization with subsequent cleavage of synaptobrevin, thus blocking synaptic vesicle fusion with the presynaptic membrane. Notably, BoNT-D is not toxic to human cells. BoNT/D precursor is 1276 amino acids (aa) in length. Following internalization and precursor proteolytic cleavage, it assumes a mature form that shows a disulfide-linked 442 aa light chain/enzyme N-terminus, and a 834 aa heavy chain/receptor-binding C-terminus. The 100 kDa heavy chain creates a channel within the endosome that allows for redox rupture of the disulfide bond and entry of the light chain into the cytosol. Covalent attachment of cargo molecules to the N-terminus is used to transport molecules into the cytosol. The D light chain shares less that 50% aa identity with the other botulinum serotype light chains.        

Long Name

Botulinum Neurotoxin Type D Light Chain

Alternate Names

BoNTD Light Chain

UniProt

P19321 (C. botulinum)

Additional BoNT-D Light Chain Products

Product Documents for C. botulinum BoNT-D Light Chain Antibody [Biotin]

Certificate of Analysis

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Product Specific Notices for C. botulinum BoNT-D Light Chain Antibody [Biotin]

For research use only

Related Research Areas

Metalloproteases and Regulators

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