Calnexin Antibody (1C2.2D11) [FITC]

Novus Biologicals | Catalog # NBP2-36570F

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (91%), Canine (92%), Primate (99%), Rat (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1C2.2D11
View all Calnexin Primary Antibodies »

Product Specifications

Immunogen

Partial recombinant human Calnexin protein (between amino acids 1-300). [UniProt P27824]

Reactivity Notes

Immunogen's sequence similarity with other species: Mouse (88%), Duck (81%), Xenopus (76%)

Localization

Endoplasmic reticulum membrane, Melanosome

Marker

Endoplasmic Reticulum Membrane Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Calnexin Antibody (1C2.2D11) [FITC]

Immunocytochemistry/Immunofluorescence: Calnexin Antibody (1C2.2D11) [FITC] [NBP2-36570F] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Calnexin (1C2.2D11) conjugated to FITC [NBP2-36570F] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow (Intracellular): Calnexin Antibody (1C2.2D11) [FITC] [NBP2-36570F] - An intracellular stain was performed on HeLa cells with Calnexin Antibody (1C2.2D11) NBP2-36570F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.
Calnexin Antibody (1C2.2D11) [FITC]

Calnexin (1C2.2D11) in U-2 OS Human Cell Line.

Calnexin (1C2.2D11) was detected in immersion fixed U-2 OS human osteosarcoma cell line using Mouse anti-Calnexin (1C2.2D11) Protein G Purified Monoclonal Antibody conjugated to FITC (Catalog # NBP2-36570F) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

Applications for Calnexin Antibody (1C2.2D11) [FITC]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Spectra Viewer

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Calnexin

Calnexin, an abundant approx. 90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its ER counterparts which have a KDEL sequence on their Cterminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulit-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Alternate Names

calnexin, CNX, IP90FLJ26570, Major histocompatibility complex class I antigen-binding protein p88, P90

Gene Symbol

CANX

Additional Calnexin Products

Product Documents for Calnexin Antibody (1C2.2D11) [FITC]

Certificate of Analysis

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Product Specific Notices for Calnexin Antibody (1C2.2D11) [FITC]

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Calnexin Antibody (1C2.2D11) [FITC]

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