Calpain 7 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-15548

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Calpain 7 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-220 of human Calpain 7 (NP_055111.1). RDHEGRYSEAVFYYKEAAQALIYAEMAGSSLENIQEKITEYLERVQALHSAVQSKSADPLKSKHQLDLERAHFLVTQAFDEDEKENVEDAIELYTEAVDLCLKTSYETADKVLQNKLKQLARQALDRAEALSEPLTKPVGKISSTSVKPKPPPVRAHFPLGANPFLERPQSFISPQSCDAQGQRYTAEEIEVLRTTSKING

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpain 7 Antibody - Azide and BSA Free

Western Blot: Calpain 7 AntibodyAzide and BSA Free [NBP3-15548]

Western Blot: Calpain 7 AntibodyAzide and BSA Free [NBP3-15548]

Western Blot: Calpain 7 Antibody [NBP3-15548] - Western blot analysis of extracts of Jurkat cells, using Calpain 7 Rabbit pAb (NBP3-15548) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Calpain 7 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Calpain 7 Antibody - Azide and BSA Free [NBP3-15548] -

Immunocytochemistry/ Immunofluorescence: Calpain 7 Antibody - Azide and BSA Free [NBP3-15548] - Immunofluorescence analysis of L929 cells using Calpain 7 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Calpain 7 Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Calpain 7 Antibody - Azide and BSA Free [NBP3-15548] -

Immunocytochemistry/ Immunofluorescence: Calpain 7 Antibody - Azide and BSA Free [NBP3-15548] - Immunofluorescence analysis of A431 cells using Calpain 7 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for Calpain 7 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Calpain 7

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The function of the protein encoded by this gene is not known. An orthologue has been found in mouse but it seems to diverge from other family members. The mouse orthologue is thought to be calcium independent with protease activity. [provided by RefSeq]

Alternate Names

calpain 7, calpain like protease, CALPAIN7, EC 3.4.22, EC 3.4.22.-, FLJ36423, homolog of Aspergillus Nidulans PALB, PalB homolog, PalBH, PALBHcalpain-7

Gene Symbol

CAPN7

Additional Calpain 7 Products

Product Documents for Calpain 7 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Calpain 7 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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