CAPZA1 Antibody (OTI2G4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70336

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2G4

Format

Azide and BSA Free
View all CAPZA1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CAPZA1(NP_006126) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

32.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CAPZA1 Antibody (OTI2G4) - Azide and BSA Free

Western Blot: CAPZA1 Antibody (OTI2G4)Azide and BSA Free [NBP2-70336]

Western Blot: CAPZA1 Antibody (OTI2G4)Azide and BSA Free [NBP2-70336]

Western Blot: CAPZA1 Antibody (OTI2G4) - Azide and BSA Free [NBP2-70336] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CAPZA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPZA1.
Immunohistochemistry: CAPZA1 Antibody (OTI2G4) - Azide and BSA Free [NBP2-70336]

Immunohistochemistry: CAPZA1 Antibody (OTI2G4) - Azide and BSA Free [NBP2-70336]

Immunohistochemistry: CAPZA1 Antibody (OTI2G4) - Azide and BSA Free [NBP2-70336] - Staining of paraffin-embedded pancreas tissue using anti-CAPZA1 mouse monoclonal antibody.
Immunohistochemistry: CAPZA1 Antibody (OTI2G4) - Azide and BSA Free [NBP2-70336]

Immunohistochemistry: CAPZA1 Antibody (OTI2G4) - Azide and BSA Free [NBP2-70336]

Immunohistochemistry: CAPZA1 Antibody (OTI2G4) - Azide and BSA Free [NBP2-70336] - Staining of paraffin-embedded liver tissue using anti-CAPZA1 mouse monoclonal antibody.
Immunohistochemistry: CAPZA1 Antibody (OTI2G4) - Azide and BSA Free [NBP2-70336]

Immunohistochemistry: CAPZA1 Antibody (OTI2G4) - Azide and BSA Free [NBP2-70336]

Immunohistochemistry: CAPZA1 Antibody (OTI2G4) - Azide and BSA Free [NBP2-70336] - Staining of paraffin-embedded prostate tissue using anti-CAPZA1 mouse monoclonal antibody.

Applications for CAPZA1 Antibody (OTI2G4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:50

Western Blot

1:500

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CAPZA1

CAPZA1 is a member of the F-actin capping protein alpha subunit family. This gene encodes the alpha subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments, and has roles in cell motility and actin assembly

Alternate Names

Cap Z, CAPPA1, capping protein (actin filament) muscle Z-line, alpha 1, CAPZ, CapZ alpha-1, CAZ1, F-actin-capping protein subunit alpha-1

Gene Symbol

CAPZA1

Additional CAPZA1 Products

Product Documents for CAPZA1 Antibody (OTI2G4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CAPZA1 Antibody (OTI2G4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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