Carbonic Anhydrase I/CA1 Antibody (24GB3680)

Novus Biologicals | Catalog # NBP3-50729

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 24GB3680
View all Carbonic Anhydrase I/CA1 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Carbonic Anhydrase I/CA1 (Uniprot: P00915)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carbonic Anhydrase I/CA1 Antibody (24GB3680)

Carbonic Anhydrase I/CA1 Antibody (24GB3680)

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase I/CA1 Antibody (24GB3680)[NBP3-50729] -

Immunocytochemical staining of H9c2 cells with Carbonic Anhydrase I/CA1 antibody(1:1,000). Nuclei were stained blue with DAPI; Carbonic Anhydrase I/CA1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 um.
Carbonic Anhydrase I/CA1 Antibody (24GB3680)

Flow Cytometry: Carbonic Anhydrase I/CA1 Antibody (24GB3680)[NBP3-50729] -

Flow cytometric analysis of Carbonic Anhydrase I/CA1expression in H9c2 cells using Carbonic Anhydrase I/CA1 antibody (1:2,000). Green, isotype control; red, Carbonic anhydrase 1.
Carbonic Anhydrase I/CA1 Antibody (24GB3680)

Western Blot: Carbonic Anhydrase I/CA1 Antibody (24GB3680)[NBP3-50729] -

Western blotting analysis using Carbonic Anhydrase I/CA1 antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for Carbonic Anhydrase I/CA1 Antibody (24GB3680)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase I/CA1

Carbonic anhydrase performs an important role in respiration by facilitating transfer of CO2. Carbonic Anhydrase I (CAI) is a single polypeptide chain metalloenzyme of molecular weight 29 kDa. It is present mainly in erythrocytes at a concentration of about 12 mg per gram haemoglobin. Its main enzyme function is to catalyse the reversible hydration of carbon dioxide. It also catalyses the hydrolysis of certain esters. The twelve active CA isozymes are thought to regulate a variety of cellular functions including several processes in the reproductive systems. A mutational deficiency has been described but patients exhibit no adverse chemical symptoms. Red cell CAI levels are decreased in patients with thyrotoxicosis.

Alternate Names

CA1

Gene Symbol

CA1

Additional Carbonic Anhydrase I/CA1 Products

Product Documents for Carbonic Anhydrase I/CA1 Antibody (24GB3680)

Certificate of Analysis

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Product Specific Notices for Carbonic Anhydrase I/CA1 Antibody (24GB3680)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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