Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488]

Novus Biologicals | Catalog # NBP2-89518AF488

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 039 expressed in HEK293
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Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human Carbonic Anhydrase IV/CA4 (Accession#: NP_000708.1; Met1-Lys283).

Specificity

No cross-reactivity in ELISA with: Human CA2Human CA3Human CA5AHuman CA5BHuman CA8Human CA9Human CA10Human CA12Human CA13Human CA14

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488]

Flow Cytometry: Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488] [NBP2-89518AF488] - Human primary renal tubular epithelial cells stained with carbonic Anhydrase IV/CA4 antibody conjugated to Alexa Fluor 488. Cells were immediately fixed with 4% PFA after detachment from culture plates and prior to staining. Final Ab concentration was 1:50. Image from verified customer review.

Applications for Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. Carbonic Anhydrase IV/CA4 Antibody validated for Flow Cytometry from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-89518AF488 in the following applications:

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Carbonic Anhydrase IV/CA4

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq]

Alternate Names

CA4, RP17

Gene Symbol

CA4

Additional Carbonic Anhydrase IV/CA4 Products

Product Documents for Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488] (1)

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Customer Images


Showing  1 - 1 of 1 review Showing All
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  • Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488]
    Name: Sean DeWolf
    Application: Flow Cytometry
    Sample Tested: Renal tubule
    Species: Human
    Verified Customer | Posted 10/31/2022
    Human primary renal tubular epithelial cells stained with carbonic anhydrase 4 Ab conjugated to Alexa Fluor 488. Cells were immediately fixed with 4% PFA after detachment from culture plates and prior to staining. Final Ab concentration was 1:50.
    Carbonic Anhydrase IV/CA4 Antibody (039) [Alexa Fluor® 488] NBP2-89518AF488
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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