Carbonic Anhydrase XII/CA12 Antibody (CL0280) - BSA Free

Novus Biologicals | Catalog # NBP2-52902

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0280

Format

BSA Free
View all Carbonic Anhydrase XII/CA12 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O43570, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - BSA Free

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Western Blot: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Lane 1: Marker [kDa], Lane 2: Human RT-4 cell line
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Staining of human pancreas shows strong membranous and moderate cytopalsmic positivity in the exocrine cells.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Staining of human colon shows strong cytoplasmic and membrane positivity in glandular cells.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Staining of human kidney (renal cancer) shows moderate immunoreactivity in cancer cells.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody (CL0280) [NBP2-52902] - Staining of human kidney shows strong membranous and/or moderate cytoplasmic immunoreactivity in subsets of renal tubules, while glomeruli are negative.

Applications for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase XII/CA12

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.

Alternate Names

CA12

Gene Symbol

CA12

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - BSA Free

Certificate of Analysis

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Product Specific Notices for Carbonic Anhydrase XII/CA12 Antibody (CL0280) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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