Carbonic Anhydrase XII/CA12 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81668

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Carbonic Anhydrase XII/CA12 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQ

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (88%). Reactivity reported in scientific literature (PMID: 22016510)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Carbonic Anhydrase XII/CA12 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668]

Immunocytochemistry/ Immunofluorescence: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668]

Immunocytochemistry/Immunofluorescence: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668] - Staining of human cell line RT4 shows localization to nucleus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668]

Immunohistochemistry-Paraffin: Carbonic Anhydrase XII/CA12 Antibody [NBP1-81668] - Staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.

Applications for Carbonic Anhydrase XII/CA12 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

Reported in (PMID: 26911677).
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Carbonic Anhydrase XII/CA12

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.

Alternate Names

CA12

Gene Symbol

CA12

Additional Carbonic Anhydrase XII/CA12 Products

Product Documents for Carbonic Anhydrase XII/CA12 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Carbonic Anhydrase XII/CA12 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Carbonic Anhydrase XII/CA12 Antibody - BSA Free

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Protocols

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