Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2D11

Format

Azide and BSA Free
View all Carboxypeptidase M Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 124-423 of human CPM(NP_001865) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

48.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free (NBP2-46352) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free

Western Blot: Carboxypeptidase M Antibody (OTI2D11)Azide and BSA Free [NBP2-70446]

Western Blot: Carboxypeptidase M Antibody (OTI2D11)Azide and BSA Free [NBP2-70446]

Western Blot: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CPM ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti CPM.(1:2000).
Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446]

Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446]

Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CPM mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,)(1:150).
Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446]

Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446]

Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CPM mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min) (1:150)
Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446]

Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446]

Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CPM mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,)(1:150)
Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446]

Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446]

Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CPM mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,)(1:150)
Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446]

Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446]

Immunohistochemistry: Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free [NBP2-70446] - Staining of paraffin-embedded Human liver tissue within the normal limits using anti CPM mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)

Applications for Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carboxypeptidase M

The protein encoded by the Carboxypeptidase M gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Alternate Names

CPM

Gene Symbol

CPM

Additional Carboxypeptidase M Products

Product Documents for Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Carboxypeptidase M Antibody (OTI2D11) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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