CARD11/CARMA1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92910

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CARD11/CARMA1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 985-1154 of human CARD11 (NP_115791.3). AKTLVQRLLNSGGAMEFTICKSDIVTRDEFLRRQKTETIIYSREKNPNAFECIAPANIEAVAAKNKHCLLEAGIGCTRDLIKSNIYPIVLFIRVCEKNIKRFRKLLPRPETEEEFLRVCRLKEKELEALPCLYATVEPDMWGSVEELLRVVKDKIGEEQRKTIWVDEDQL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

133 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CARD11/CARMA1 Antibody - BSA Free

Western Blot: CARD11/CARMA1 AntibodyBSA Free [NBP2-92910]

Western Blot: CARD11/CARMA1 AntibodyBSA Free [NBP2-92910]

Western Blot: CARD11/CARMA1 Antibody [NBP2-92910] - Analysis of extracts of various cell lines, using CARD11/CARMA1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.
CARD11/CARMA1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CARD11/CARMA1 Antibody - BSA Free [NBP2-92910] -

Immunocytochemistry/ Immunofluorescence: CARD11/CARMA1 Antibody - BSA Free [NBP2-92910] - Immunofluorescence analysis of A549 cells using CARD11/CARMA1 antibody. Blue: DAPI for nuclear staining.

Applications for CARD11/CARMA1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CARD11/CARMA1

CARD11 belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10.

Long Name

Caspase Recruitment Domain-containing Protein 11

Alternate Names

BIMP3, CARMA1

Gene Symbol

CARD11

Additional CARD11/CARMA1 Products

Product Documents for CARD11/CARMA1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CARD11/CARMA1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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