Caspase-14 Antibody (70A1426) - BSA Free

Novus Biologicals | Catalog # NB100-56718

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 70A1426

Format

BSA Free
View all Caspase-14 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised against a synthetic peptide corresponding to amino acids 2-18 of mouse Caspase-14.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Caspase-14 Antibody (70A1426) - BSA Free

Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718]

Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718]

Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis of Caspase 14 in mouse spleen lysate using Caspase 14 antibody at 1:1000. Novus's goat anti-mouse Ig HRP secondary antibody (Novus, 20101) and PicoTect ECL substrate solution (Novus, 10087K) were used for this test.
Flow Cytometry: Caspase-14 Antibody (70A1426) [NB100-56718]

Flow Cytometry: Caspase-14 Antibody (70A1426) [NB100-56718]

Flow Cytometry: Caspase-14 Antibody (70A1426) [NB100-56718] - Intracellular analysis of Caspase-14 in 10^6 Jurkat cells probed with 1:500 dilution of Caspase-14 antibody. Shaded histogram represents cells without antibody; green represents isotype control antibody; red represents Caspase-14 antibody. This antibody was used for this test, and an anti-mouse IgG FITC conjugated secondary antibody.
Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718]

Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718]

Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis of human breast cancer tissue using Caspase 14 antibody at a concentration of 0.1 ug/ml.
Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718]

Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718]

Western Blot: Caspase-14 Antibody (70A1426) [NB100-56718] - Analysis for Caspase-14 using NB100-56718 at 2 ug/ml dilution against 10 ug of mouse spleen cell lysate.

Applications for Caspase-14 Antibody (70A1426) - BSA Free

Application
Recommended Usage

Flow Cytometry

(Intracellular): 1:500/10^6 cells

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 18520823)

Western Blot

0.1-4 ug/ml
Application Notes
In mouse spleen, a 30 kDa band is observed.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites with PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-14

Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase-14 is a newly identified member of Caspase family. It has conserved active site pentapeptide, QACRG. However, no proteolytic or biological activities have been identified so far. It's high expression in embryonic tissues and limited expression in adult tissues suggests that it may have some role during ontogenesis.

Alternate Names

CASP14, Caspase14

Entrez Gene IDs

12365 (Mouse)

Gene Symbol

CASP14

UniProt

NP_033939 (Mouse)

Additional Caspase-14 Products

Product Documents for Caspase-14 Antibody (70A1426) - BSA Free

Certificate of Analysis

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Product Specific Notices for Caspase-14 Antibody (70A1426) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

Citations for Caspase-14 Antibody (70A1426) - BSA Free

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Protocols

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FAQs

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