Caspase-4 Antibody (7G7) - Azide and BSA Free

Novus Biologicals | Catalog # H00000837-M02-100ug

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 7G7

Format

Azide and BSA Free
View all Caspase-4 Primary Antibodies »

Product Specifications

Immunogen

CASP4 (AAH17839, 1 a.a. ~ 377 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAEGNHRKKPLKVLESLGKDFLTGVLDNLVEQNVLNWKEEEKKKYYDAKTEDKVRVMADSMQEKQRMAGQMLLQTFFNIDQISPNKKAHPNMEAGPPESGESTDALKLCPHEEFLRLCKERAEEIYPIKERNNRTRLALIICNTEFDHLPPRNGADFDITGMKELLEGLDYSVDVEENLTARDMESALRAFATRPEHKSSDSTFLVLMSHGILEGICGTVHDEKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGANRGELWVRDSPASLEVASSQSSENLEEDAVYKTHVEKDFIAFCSSTPHNVSWRDSTMGSIFITQLITCFQKYSWCCHLEEVFRKVQQSFETPRAKAQMPTIERLSMTRYFYLFPGN

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Caspase-4 Antibody (7G7) - Azide and BSA Free

Western Blot: Caspase-4 Antibody (7G7) [H00000837-M02-100ug]

Western Blot: Caspase-4 Antibody (7G7) [H00000837-M02-100ug]

Western Blot: Caspase-4 Antibody (7G7) [H00000837-M02-100ug] - Detection against Immunogen (67.21 KDa).
Western Blot: Caspase-4 Antibody (7G7) [H00000837-M02-100ug]

Western Blot: Caspase-4 Antibody (7G7) [H00000837-M02-100ug]

Western Blot: Caspase-4 Antibody (7G7) [H00000837-M02-100ug] - Analysis of CASP4 expression in transfected 293T cell line by CASP4 monoclonal antibody (M02), clone 7G7. Lane 1: CASP4 transfected lysate (Predicted MW: 43.3 KDa). Lane 2: Non-transfected lysate.
Western Blot: Caspase-4 Antibody (7G7) [H00000837-M02-100ug]

Western Blot: Caspase-4 Antibody (7G7) [H00000837-M02-100ug]

Western Blot: Caspase-4 Antibody (7G7) [H00000837-M02-100ug] - Analysis of CASP4 expression in HeLa.
ELISA: Caspase-4 Antibody (7G7) [H00000837-M02-100ug]

ELISA: Caspase-4 Antibody (7G7) [H00000837-M02-100ug]

ELISA: Caspase-4 Antibody (7G7) [H00000837-M02-100ug] - Detection limit for recombinant GST tagged CASP4 is 0.03 ng/ml as a capture antibody.

Applications for Caspase-4 Antibody (7G7) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Caspase-4

Caspase 4 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.

Alternate Names

CASP4, Caspase4, ICE(rel)II, ICH-2, Mih1/TX, TX

Entrez Gene IDs

837 (Human)

Gene Symbol

CASP4

OMIM

602664 (Human)

UniProt

AAH17839 (Human)

Additional Caspase-4 Products

Product Documents for Caspase-4 Antibody (7G7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caspase-4 Antibody (7G7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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