Caspase-7 Antibody (10-1-62) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-19230

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Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 10-1-62

Format

Azide and BSA Free
View all Caspase-7 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Caspase-7 protein (full length)

Reactivity Notes

May react with Hamster. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Specificity

Caspase-7 (10-1-62)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Caspase-7 Antibody (10-1-62) - Azide and BSA Free

Western Blot: Caspase-7 Antibody (10-1-62) [NBP1-19230]

Western Blot: Caspase-7 Antibody (10-1-62) [NBP1-19230]

Western Blot: Caspase-7 Antibody (10-1-62) [NBP1-19230] - Analysis of human Jurkat and Jurkat induced cell lysates probed with AAM-137
Immunohistochemistry: Caspase-7 Antibody (10-1-62) [NBP1-19230]

Immunohistochemistry: Caspase-7 Antibody (10-1-62) [NBP1-19230]

Immunohistochemistry: Caspase-7 Antibody (10-1-62) [NBP1-19230] - Human spleen tissue stained with Caspase-7, mAb (10-1-62) at 10ug/ml.
Immunohistochemistry: Caspase-7 Antibody (10-1-62) [NBP1-19230]

Immunohistochemistry: Caspase-7 Antibody (10-1-62) [NBP1-19230]

Immunohistochemistry: Caspase-7 Antibody (10-1-62) [NBP1-19230] - Human colon tissue stained with Caspase-7, mAb (10-1-62) at 10ug/ml.

Applications for Caspase-7 Antibody (10-1-62) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, pH 7.2, containing 10mM sodium phosphate, 50mM sodium chloride, and 0.1mM PMSF

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Caspase-7

Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse.

Alternate Names

CASP7, Caspase7, Mch3

Entrez Gene IDs

840 (Human); 12369 (Mouse); 64026 (Rat)

Gene Symbol

CASP7

Additional Caspase-7 Products

Product Documents for Caspase-7 Antibody (10-1-62) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Caspase-7 Antibody (10-1-62) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail