Caspase-8 Antibody (FLICE 4-1-20) - BSA Free

Novus Biologicals | Catalog # NB500-208

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # FLICE 4-1-20

Format

BSA Free
View all Caspase-8 Primary Antibodies »

Product Specifications

Immunogen

Full-length recombinant human Caspase 8 [UniProt# Q14790]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29069785).

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Caspase-8 Antibody (FLICE 4-1-20) - BSA Free

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Caspase-8-Antibody-FLICE-4-1-20-Western-Blot-NB500-208-img0004.jpg
Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208]

Western Blot: Caspase 8 Antibody (FLICE 4-1-20) [NB500-208] - Detection of Caspase 8 (59 kDa) from HEK293 cell extract using NB500-208 (1:500). Lanes 1 and 2 contain inactive and active Caspase 8, respectively.

Applications for Caspase-8 Antibody (FLICE 4-1-20) - BSA Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
This Caspase 8 (FLICE 4-1-20) antibody is useful for Western Blot where a band is seen at 55 kDa representing the inactive form of Caspase 8 (see protocol for additional information). We also have reports that the antibody detects the active (pro) form of Caspase 8.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Caspase-8

Caspases are evolutionary conserved family of aspartate-specific cystein proteases which are essential part of apoptotic machinery and typically signal in a two-step cascade: 'initiator caspases' with large prodomains (such as caspase-1, -8 and -9) are first recruited into large protein complexes in which they undergo proximity-induced autoactivation and "executioner caspases" such as caspase-3 and -6, caspase-7 which are activated by initiator caspases via freeing of executioner caspases of their short inhibitory prodomain, allowing them to cleave a large set of cellular substrates. Localized in cytoplasm, caspase 7 is a heterotetramer that is consists of two anti-parallel arranged heterodimers, each one formed by a 20 kD (p20) and a 11 kD (p11) subunit, and interacts with BIRC6/bruce. Caspase 7 cleaves/activates SREBPs and poly(ADP-ribose) polymerase (PARP). Its overexpression promotes apoptosis, and it also implicates in inflammation signaling. Therapeutic intervention with caspase-7 has been shown to prove beneficial in conditions where excessive cell death and/or inflammation contribute to diseases e.g. Alzheimer's disease, Huntington's disease, sepsis, rheumatoid arthritis, cancer and NIDDM.

Alternate Names

CASP8, Caspase8, Mch5

Entrez Gene IDs

841 (Human)

Gene Symbol

CASP8

UniProt

Q14790

Additional Caspase-8 Products

Product Documents for Caspase-8 Antibody (FLICE 4-1-20) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Caspase-8 Antibody (FLICE 4-1-20) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Caspase-8 Antibody (FLICE 4-1-20) - BSA Free

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Protocols

View specific protocols for Caspase-8 Antibody (FLICE 4-1-20) - BSA Free (NB500-208):

Caspase-8 Antibody (FLICE 4-1-20):
Western Protocol

1) Run a 10-*15% SDS polyacrylamide gel, loading ~20 ug of cell extract per lane.
2) Transfer the proteins to a membrane.
3) Block the membrane in PT-T20 [20 mM Tris-HCl, pH 7.4 / 150 mM NaCl/0.5% Tween 20] + 5% NFDM [non-fat dry milk], for 3 hour at room temperature (RT), shaking gently.
4) Rinse the membrane twice with PT-T20.
5) Incubate the membrane in anti-Caspase 8 [NB 500-208], diluted 1:500-1:1,000 in PT-T20 + 5% NFDM, for 60 minutes at RT.
6) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
7) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in PT-T20 + 5% NFDM for 60 minutes at RT.
8) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
9) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.


*Positive control(s): Human kidney 293 cells
Western Protocol

1) Run a 10-*15% SDS polyacrylamide gel, loading ~20 ug of cell extract per lane.
2) Transfer the proteins to a membrane.
3) Block the membrane in PT-T20 [20 mM Tris-HCl, pH 7.4 / 150 mM NaCl/0.5% Tween 20] + 5% NFDM [non-fat dry milk], for 3 hour at room temperature (RT), shaking gently.
4) Rinse the membrane twice with PT-T20.
5) Incubate the membrane in anti-Caspase 8 [NB 500-208], diluted 1:500-1:1,000 in PT-T20 + 5% NFDM, for 60 minutes at RT.
6) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
7) Incubate the membrane in anti-mouse conjugated to HRP (secondary antibody), diluted in PT-T20 + 5% NFDM for 60 minutes at RT.
8) Wash the membrane for 15 minutes, 3 times, in PT-T20 at RT.
9) Develop membrane in chemiluminescent reagents, as instructed by kit-vendor.


*Positive control(s): Human kidney 293 cells

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Caspase-8 Antibody (FLICE 4-1-20) - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Does NB500-208 both recognize human and rat active form of caspase 8 (subunit p18 or subunit p10)? Besides that please let me know if NB500-208 also recognize pro-form caspase 8?

    A: Caspase 8 Antibody (FLICE 4-1-20) # NB500-208 antibody does not recognize rat samples. It is human specific and in our QC data it was successfully tested to detect the pro or inactive form of caspase at molecular weight 59kd. However, depending upon the extent of extrinsic apoptotic pathway activation in your cells or tissues, you can expect to see bands of the active form also.

  • Q: May we ask if NB600-1343, NB100-56527 and NB500-208 can recognize both active form and profor

    A: Product NB100-56527 recognises both pro and active forms of Caspase 8. NB500-208 recognises only the active form, and NB600-1343 has not been tested for specificity at this time.

  • Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

    A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

  • Q: Does NB500-208 both recognize human and rat active form of caspase 8 (subunit p18 or subunit p10)? Besides that please let me know if NB500-208 also recognize pro-form caspase 8?

    A: Caspase 8 Antibody (FLICE 4-1-20) # NB500-208 antibody does not recognize rat samples. It is human specific and in our QC data it was successfully tested to detect the pro or inactive form of caspase at molecular weight 59kd. However, depending upon the extent of extrinsic apoptotic pathway activation in your cells or tissues, you can expect to see bands of the active form also.

  • Q: May we ask if NB600-1343, NB100-56527 and NB500-208 can recognize both active form and profor

    A: Product NB100-56527 recognises both pro and active forms of Caspase 8. NB500-208 recognises only the active form, and NB600-1343 has not been tested for specificity at this time.

  • Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

    A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

  • Q: Does NB500-208 both recognize human and rat active form of caspase 8 (subunit p18 or subunit p10)? Besides that please let me know if NB500-208 also recognize pro-form caspase 8?

    A: Caspase 8 Antibody (FLICE 4-1-20) # NB500-208 antibody does not recognize rat samples. It is human specific and in our QC data it was successfully tested to detect the pro or inactive form of caspase at molecular weight 59kd. However, depending upon the extent of extrinsic apoptotic pathway activation in your cells or tissues, you can expect to see bands of the active form also.

  • Q: May we ask if NB600-1343, NB100-56527 and NB500-208 can recognize both active form and profor

    A: Product NB100-56527 recognises both pro and active forms of Caspase 8. NB500-208 recognises only the active form, and NB600-1343 has not been tested for specificity at this time.

  • Q: May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?

    A: In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.

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Associated Pathways

Apoptosis Signaling Pathway Apoptosis Signaling Pathway Thumbnail