Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cathepsin A/Lysosomal Carboxypeptidase A Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human Cathepsin A/Lysosomal Carboxypeptidase A fragment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98190]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98190]

Immunohistochemistry-Paraffin: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98190] - Staining of human Cathepsin A/Lysosomal Carboxypeptidase A in human kidney with rabbit polyclonal antibody at 1:100 dilution.
Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98190] -

Immunocytochemistry/Immunofluorescence: Cathepsin A/Lysosomal Carboxypeptidase A Antibody [NBP2-98190] - Immunofluorescence staining of Cathepsin A/Lysosomal Carboxypeptidase A in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98190 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cathepsin A/Lysosomal Carboxypeptidase A

Lysosomal protective protein/cathepsin A (PPCA) is a lysosomal serine carboxypeptidase that forms an intralysosomal enzyme-complex with b-galactosidase and neuraminidase (NEU1). PPCA is synthesized as a 54-kDa precursor/zymogen, and proteolytically cleaved in the lysosome into a catalytically active 32- and 20-kDa two-chain enzyme. The enzyme has cathepsin A activity at acidic pH but maintains also a deamidase/esterase activity at neutral pH. Furthermore, the human enzyme, purified from platelets and lymphocytes, has been shown to function on the inactivation of selected neuropeptides, like substance P, oxytocin, and endothelin I. The autosomal recessive genetic deficiency of PPCA causes galactosialidosis, a neurodegenerative lysosomal storage disorder, resulting in the secondary deficiencies of b-galactosidase and NEU1.

Alternate Names

CTSA, Lysosomal Carboxypeptidase A

Gene Symbol

CTSA

Additional Cathepsin A/Lysosomal Carboxypeptidase A Products

Product Documents for Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cathepsin A/Lysosomal Carboxypeptidase A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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