Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human Core-Binding Factor, Beta Subunit using the numbering given in entry NP_001746.1 (GeneID 865).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit CBFB Antibody - BSA Free (NBP2-04107) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CBFB Antibody - BSA Free

Immunoprecipitation: CBFB Antibody [NBP2-04107]

Immunoprecipitation: CBFB Antibody [NBP2-04107]

Immunoprecipitation: CBFB Antibody [NBP2-04107] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: CBF-beta antibody NBP2-04107 used for IP at 6 ug/mg lysate. Isoform 1 and isoform 2 were also immunoprecipitated by CBF-beta antibody, which recognizes an epitope that is common to both isoform 1 and isoform 2. Isoform 1 was immunoprecipitated by CBF-beta (isoform 1) antibody NBP2-04109, and isoform 2 of CBF-beta was immunoprecipitated by CBF-beta (isoform 2) antibody NBP2-04108. For blotting immunoprecipitated CBF-beta, NBP2-04109 and NBP2-04107 were used at 1 ug/ml in A and B, respectively. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).
Immunoprecipitation: CBFB Antibody [NBP2-04107]

Immunoprecipitation: CBFB Antibody [NBP2-04107]

Immunoprecipitation: CBFB Antibody [NBP2-04107] - Detection of human CBF-beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CBF-beta antibody NBP2-04107 (lot NBP2-04107-2) used for IP at 6 ug per reaction. CBF-beta was also immunoprecipitated by a previous lot of this antibody (lot NBP2-04107-1). For blotting immunoprecipitated CBF-beta, rabbit anti-CBF-beta antibody was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for CBFB Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

Not recommended

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CBFB

The transcription factor Polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (Osteoblast-specific transcription factor), CBFA1 (Core Binding Factor) and AML3 (Acute myeloid leukemia), is composed of two subunits, alpha and beta, which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2alpha subunits, PEBP2alphaA, PEBP2alphaB and PEBP2alphaC, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, runt. This region is involved in DNA binding and heterodimerization with the regulatory beta subunit, which facilitates DNA binding of the alpha subunit. Both subunits are required for in vivo function; the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2beta is the target of chromosomal inversion 16 (p13;q22) with the smooth muscle myosin heavy chain, producing a chimeric gene, PEBP2beta/CBFbeta-SMMHC, that is associated with human acute myeloid leukemia.

Long Name

Core-binding Factor Subunit beta

Alternate Names

CBF-beta, PEA2-beta, PEBP2B, SL3-3

Gene Symbol

CBFB

UniProt

NP_001746.1 (Human)

Additional CBFB Products

Product Documents for CBFB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CBFB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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