CBX3 Antibody (7B7Q2)

Novus Biologicals | Catalog # NBP3-16368

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7B7Q2 expressed in HEK293
View all CBX3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CBX3 (NP_057671.2). MASNKTTLQKMGKKQNGKSKKVEEAEPEEFVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEAFLNSQKAGKEKDGTKRKSLSDSESD

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CBX3 Antibody (7B7Q2)

Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368]

Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368]

Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368] - Western blot analysis of extracts of various cell lines, using CBX3 Rabbit mAb (NBP3-16368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Immunohistochemistry-Paraffin: CBX3 Antibody (7B7Q2) [NBP3-16368]

Immunohistochemistry-Paraffin: CBX3 Antibody (7B7Q2) [NBP3-16368]

Immunohistochemistry-Paraffin: CBX3 Antibody (7B7Q2) [NBP3-16368] - Immunohistochemistry of paraffin-embedded human colon using CBX3 Rabbit mAb (NBP3-16368) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368]

Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368]

Western Blot: CBX3 Antibody (7B7Q2) [NBP3-16368] - Western blot analysis of extracts of Rat brain, using CBX3 Rabbit mAb (NBP3-16368) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Applications for CBX3 Antibody (7B7Q2)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CBX3

HP1 gamma appears to be involved in transcriptional silencing in heterochromatin-like complexes. It recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. HP1 gamma may contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR) and involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.

Alternate Names

chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox protein homolog 3, Drosophila), HECH, Heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, HP1 gamma, HP1 gamma homolog, HP1-GAMMA, Modifier 2 protein

Gene Symbol

CBX3

Additional CBX3 Products

Product Documents for CBX3 Antibody (7B7Q2)

Certificate of Analysis

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Product Specific Notices for CBX3 Antibody (7B7Q2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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