Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CBX3 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 133 and the C-terminus (residue 183) of human chromobox homolog 3 using the numbering given in entry NP_009207.2 (GeneID 11335).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-381, NB100-2350

Scientific Data Images for CBX3 Antibody - BSA Free

Immunohistochemistry: CBX3 Antibody [NB100-41429]

Immunohistochemistry: CBX3 Antibody [NB100-41429]

Immunohistochemistry: CBX3 Antibody [NB100-41429] - Sample: FFPE section of human ovarian carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-CBX3 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunoprecipitation: CBX3 Antibody [NB100-41429]

Immunoprecipitation: CBX3 Antibody [NB100-41429]

Immunoprecipitation: CBX3 Antibody [NB100-41429] - Detection of Human CBX3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBX3 antibody NB100-41429 used for IP at 3 mcg/mg lysate. CBX3 was also immunoprecipitated by rabbit anti-CBX3 antibodies NB100-41427 and NB100-41428, which recognize upstream epitopes. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for CBX3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CBX3

CBX3 (chromobox protein homolog 3), also known as HP1gamma (heterochromatin protein 1 homolog gamma), is one of 3 isoforms of HP1 that binds heterochromatin and functions as a structural adaptor to stabilize and organize macromolecular complexes with nucleosomes for higher chromatin structure. All of the HP1 isoforms possess a chromodomain and chromoshadow domain and associate with methylated histones that mediate transcriptional repression.

Alternate Names

chromobox homolog 3, chromobox homolog 3 (Drosophila HP1 gamma), chromobox protein homolog 3, Drosophila), HECH, Heterochromatin protein 1 homolog gamma, heterochromatin protein HP1 gamma, heterochromatin-like protein 1, HP1 gamma, HP1 gamma homolog, HP1-GAMMA, Modifier 2 protein

Entrez Gene IDs

11335 (Human)

Gene Symbol

CBX3

UniProt

NP_009207.2 (Human)

Additional CBX3 Products

Product Documents for CBX3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CBX3 Antibody - BSA Free

This antibody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-381, NB100-2350

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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