Cbx8 Antibody

Novus Biologicals | Catalog # NB100-96915

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Cbx8 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-RERGTSRVDDKPSS corresponding to internal region according to NP_065700.1.

Epitope

RERGTSRVDDKPSS

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Cbx8 Antibody

Western Blot: Cbx8 Antibody [NB100-96915]

Western Blot: Cbx8 Antibody [NB100-96915]

Western Blot: Cbx8 Antibody [NB100-96915] - (0.1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Cbx8 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 40 kDa band observed in K562 lysates (calculated MW of 43.4 kDa band according to NP_065700.1). An additional band of unknown identity was also consistently observed at 28 kDa. This band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cbx8

CBX8 is an essential component of one of the polycomb repressive complexes, which directly regulate the expression of numerous target genes, including the INK4A-ARF locus, involved in cell-fate decisions.

Alternate Names

chromobox homolog 8, chromobox homolog 8 (Drosophila Pc class), chromobox homolog 8 (Pc class homolog, Drosophila), hPc3, Pc3, PC3chromobox protein homolog 8, polycomb 3, Polycomb 3 homolog, RC1Pc class 3 homolog, Rectachrome 1

Entrez Gene IDs

57332 (Human); 30951 (Mouse)

Gene Symbol

CBX8

UniProt

NP_065700.1 (Human)

Additional Cbx8 Products

Product Documents for Cbx8 Antibody

Certificate of Analysis

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Product Specific Notices for Cbx8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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