CCDC114 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CCDC114 Antibody - BSA Free [NBP1-93863] -

The ODAD1 mutation (c.71-2A > C; c.598-2A > C) led to the absence of wild-type ODAD1 and the defects of the outer dynein arm in ciliary axonemes and caused a decrease in ciliary beat frequency. (A) Diagram of ODAD1 demonstrating the antigenic site of the antibody used (red box) and the location of the mutation (arrow) in the patient. Yellow boxes represent predicted coiled-coil domains, and blue boxes represent disordered domains. (B) In control individuals, ODAD1 was localized along the length of the axoneme in ciliated cells. (C) In patient Ⅲ:1, the expression of ODAD1 was significantly low. Green, acetylated-alpha -tubulin; red, ODAD1; blue, DAPI. Scale bar = 20 μm. (D) Ultrastructure of the ciliary axonemes of the patient and control individuals was analyzed via TEM; defects in the ODA of ciliary axonemes were observed in the patient. While many ciliary cross-sections did not show ODA, some sections exhibited ODAs. The white asterisks indicate the structure of ODAs (scale bar = 100 nm). (E) CBF is significantly lower in the patient than in control individuals (***, p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38028630), licensed under a CC-BY license. Not internally tested by Novus Biologicals.