CCL2/JE/MCP-1 Antibody (11k2) - Chimeric - BSA Free

Name: CCL2/JE/MCP-1 Antibody (11k2) - Chimeric - BSA Free
Brand: Novus Biologicals
SKU: NBP3-45119
Price: 649 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-45119

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Block/Neutralize

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 11k2

Format

BSA Free

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Product Specifications

Immunogen

Human CCL2/JE/MCP-1

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Scientific Data Images for CCL2/JE/MCP-1 Antibody (11k2) - Chimeric - BSA Free

Flow Cytometry: CCL2/JE/MCP-1 Antibody (11k2) - Chimeric - BSA Free [NBP3-45119] -

Flow Cytometry: CCL2/JE/MCP-1 Antibody (11k2) - Chimeric - BSA Free [NBP3-45119] - Paraformaldehyde-fixed HepG2 cells were permeabilized with 0.5% Triton and stained with the anti-unknown specificity antibody (isotype control, black line) or NBP3-45119 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunocytochemistry/ Immunofluorescence: CCL2/JE/MCP-1 Antibody (11k2) - Chimeric - BSA Free [NBP3-45119] -

Immunocytochemistry/ Immunofluorescence: CCL2/JE/MCP-1 Antibody (11k2) - Chimeric - BSA Free [NBP3-45119] - Immunofluorescence analysis of paraformaldehyde-fixed HepG2 cells on coverslips stained with NBP3-45119 (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, NBP3-45119, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody.

Applications for CCL2/JE/MCP-1 Antibody (11k2) - Chimeric - BSA Free

Application

Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Can block monocyte chemotaxis in cell migration assays

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CCL2/JE/MCP-1

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4⁺ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Gene Symbol

CCL2

Additional CCL2/JE/MCP-1 Products

Product Documents for CCL2/JE/MCP-1 Antibody (11k2) - Chimeric - BSA Free

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Certificate of Analysis

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Product Specific Notices for CCL2/JE/MCP-1 Antibody (11k2) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.