CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.
CCL2/JE/MCP-1 Antibody (238607) [DyLight 405]
Novus Biologicals | Catalog # FAB2137E
Key Product Details
Species Reactivity
Cotton Rat
Applications
Western Blot
Label
DyLight 405 (Excitation = 400 nm, Emission = 420 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 238607
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Product Specifications
Immunogen
E. coli-derived recombinant cotton rat CCL2/JE/MCP‑1
Gln25-Asn150
Accession # AAN85636
Gln25-Asn150
Accession # AAN85636
Specificity
Detects cotton rat CCL2/JE/MCP‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse CCL2 is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8,
11, 13, 14, 15, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1,
3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20
is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for CCL2/JE/MCP-1 Antibody (238607) [DyLight 405]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CCL2/JE/MCP-1
Alternate Names
GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF
Gene Symbol
CCL2
Additional CCL2/JE/MCP-1 Products
Product Documents for CCL2/JE/MCP-1 Antibody (238607) [DyLight 405]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CCL2/JE/MCP-1 Antibody (238607) [DyLight 405]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
