Key Product Details

Species Reactivity

Cotton Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 238607
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Product Specifications

Immunogen

E. coli-derived recombinant cotton rat CCL2/JE/MCP‑1
Gln25-Asn150
Accession # AAN85636

Specificity

Detects cotton rat CCL2/JE/MCP‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse CCL2 is observed and no cross-reactivity with recombinant human CCL1, 2, 3, 5, 7, 8, 11, 13, 14, 15, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, recombinant mouse CCL1, 3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22, 24, 25, or recombinant rat CCL20 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Cotton Rat CCL2/JE/MCP‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Cotton Rat CCL2/JE/MCP‑1

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL2/JE/MCP-1

CCL2, also known as JE or MCP-1 (monocyte chemoattractant protein-1), is a CC chemokine produced by fibroblasts, macrophages, astrocytes, mast cells, endothelial cells and osteoblasts. It functions as a chemoattractant through ligations with CCR2 on monocytes, macrophages and lymphocytes.

Alternate Names

GDCF-2, HC11, HSMCR30, MCAF, MCP-1, SMC-CF

Entrez Gene IDs

6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)

Gene Symbol

CCL2

UniProt

Additional CCL2/JE/MCP-1 Products

Product Documents for Cotton Rat CCL2/JE/MCP‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Cotton Rat CCL2/JE/MCP‑1 Antibody

For research use only

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