CCL8/MCP-2 Antibody (CCL8/3683) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-14173

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # CCL8/3683

Format

Azide and BSA Free
View all CCL8/MCP-2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant CCL8/MCP-2 protein fragment (around aa1-99) (exact sequence is proprietary) (Uniprot: P80075)

Localization

Secreted

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-13770).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for CCL8/MCP-2 Antibody (CCL8/3683) - Azide and BSA Free

CCL8/MCP-2 Antibody (CCL8/3683) - Azide and BSA Free

CCL8/MCP-2 Antibody (CCL8/3683)

Formalin-fixed, paraffin-embedded human adrenal gland stained with CCL8/MCP-2 antibody (CCL8/3683). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
CCL8/MCP-2 Antibody (CCL8/3683) - Azide and BSA Free

Flow Cytometry: Mouse CCL8/MCP-2 mAb (CCL8/3683) - Azide and BSA Free [NBP3-14173]

Flow Cytometry: Mouse CCL8/MCP-2 mAb (CCL8/3683) - Azide and BSA Free [NBP3-14173] - 100.000 NK cells were fixed and permeabilized before staining with anti-CCL8 for 20 min at room temperature. A secondary antibody anti-mouse IgG conjugated with AF647 was used to detect CCL8+ NK cells by flow cytometry. Image from a verified customer review.
CCL8/MCP-2 Antibody (CCL8/3683) - Azide and BSA Free

Flow Cytometry: Mouse CCL8/MCP-2 mAb (CCL8/3683) - Azide and BSA Free [NBP3-14173]

Flow Cytometry: Mouse CCL8/MCP-2 mAb (CCL8/3683) - Azide and BSA Free [NBP3-14173] - NK cells were isolated and stimulated with target cells in an effector: target ratio of 2:1. Left graph depicts unstimulated cells, stimulated cells on the right. Image from a verified customer review. The customer used the PE-conjugated version of NBP3-14173, which is listed under NBP3-14173PE.

Applications for CCL8/MCP-2 Antibody (CCL8/3683) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

Validated for flow cytometry from a verified customer review.

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Reviewed Applications

Read 1 review rated 4 using NBP3-14173 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CCL8/MCP-2

MCP2 and MCP3 are two recently identified monocyte chemotactic proteins produced by human MG63 osteosarcoma cells. Both MCP2 and MCP3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP1. MCP3 also shares 58% amino acid identity with MCP2. Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4+ and CD8+ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Alternate Names

MCP-2, MCP2

Gene Symbol

CCL8

Additional CCL8/MCP-2 Products

Product Documents for CCL8/MCP-2 Antibody (CCL8/3683) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CCL8/MCP-2 Antibody (CCL8/3683) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CCL8/MCP-2 Antibody (CCL8/3683) - Azide and BSA Free (1)

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  • Test of NBP3-14173 by Flow Cytometry
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peripheral blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 03/05/2025
    100.000 NK cells were fixed and permeabilized before staining with anti-CCL8 for 20 min at room temperature. A secondary antibody anti-mouse IgG conjugated with AF647 was used to detect CCL8+ NK cells by flow cytometry.
    CCL8/MCP-2 Antibody (CCL8/3683) - Azide and BSA Free NBP3-14173
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