CCNK Antibody (3B7-1B9) - Azide and BSA Free

Novus Biologicals | Catalog # H00008812-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B7-1B9

Format

Azide and BSA Free
View all Cyclin K/CCNK Primary Antibodies »

Product Specifications

Immunogen

CCNK (AAH15935, 1 a.a. ~ 354 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKENKENSSPSVTSANLDHTKPCWYWDKKDLAHTPSQLEGLDPATEARYRREGARFIFDVGTRLGLHYDTLATGIIYFHRFYMFHSFKQFPRYVTGACCLFLAGKVEETPKKCKDIIKTARSLLNDVQFGQFGDDPKEEVMVLERILLQTIKFDLQVEHPYQFLLKYAKQLKGDKNKIQKLVQMAWTFVNDSLCTTLSLQWEPEIIAVAVMYLAGRLCKFEIQEWTSKPMYRRWWEQFVQDVPVDVLEDICHQILDLYSQGKQQMPHHTPHQLQQPPSLQPTPQVPQVQQSQPSQSSEPSQPQQKDPLILLQGWACRQPATHLLPSPLEDSLLCPRPFPHPACLQLGGWGGQPG

Specificity

CCNK - cyclin K

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CCNK Antibody (3B7-1B9) - Azide and BSA Free

Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01]

Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01]

Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01] - CCNK monoclonal antibody (M01), clone 3B7-1B9. Analysis of CCNK expression in PRC/PRF/5.
Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01]

Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01]

Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01] - Analysis of CCNK over-expressed 293 cell line, cotransfected with CCNK Validated Chimera RNAi ( Cat # H00008812-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CCNK monoclonal antibody (M01), clone 3B7-1B9 (Cat # H00008812-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01]

Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01]

Western Blot: CCNK Antibody (3B7-1B9) [H00008812-M01] - Analysis of CCNK expression in transfected 293T cell line by CCNK monoclonal antibody (M01), clone 3B7-1B9.Lane 1: CCNK transfected lysate(41 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: CCNK Antibody (3B7-1B9) [H00008812-M01] - Detection limit for recombinant GST tagged CCNK is approximately 0.03ng/ml as a capture antibody.

Applications for CCNK Antibody (3B7-1B9) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been used for RNAi validaton and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cyclin K/CCNK

The protein encoded by this gene is a member of the transcription cyclin family. These cyclins may regulate transcription through their association with and activation of cyclin-dependent kinases (CDK) that phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II. This gene product may play a dual role in regulating CDK and RNA polymerase II activities.

Long Name

Cyclin K

Alternate Names

CCNK, CPR4, IDDHDF

Entrez Gene IDs

8812 (Human)

Gene Symbol

CCNK

OMIM

603544 (Human)

UniProt

AAH15935 (Human)

Additional Cyclin K/CCNK Products

Product Documents for CCNK Antibody (3B7-1B9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CCNK Antibody (3B7-1B9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CCNK Antibody (3B7-1B9) - Azide and BSA Free

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