CCR1 Antibody (002) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-89234
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 002 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human CCR1.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CCR1 Antibody (002) - Azide and BSA Free
Western Blot: CCR1 Antibody (002) [NBP2-89234]
Western Blot: CCR1 Antibody (002) [NBP2-89234] - Lane A: HEK293 Whole Cell Lysate Lane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:49 kDaImmunoprecipitation: CCR1 Antibody (002) [NBP2-89234]
Immunoprecipitation: CCR1 Antibody (002) [NBP2-89234] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate2 uL anti-CCR1/CD191 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CCR1/CD191 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size: 43 kDaApplications for CCR1 Antibody (002) - Azide and BSA Free
Application
Recommended Usage
Immunoprecipitation
1-4 ul/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CCR1
Alternate Names
CCR1, CD191
Gene Symbol
CCR1
Additional CCR1 Products
Product Documents for CCR1 Antibody (002) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CCR1 Antibody (002) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars