CCR1 Antibody (DMC465) - Chimeric

Novus Biologicals | Catalog # NBP3-48348

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # DMC465
View all CCR1 Primary Antibodies »

Product Specifications

Immunogen

CCR1 recombinant protein expressed in mammalian cells (Accession# P32246). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for CCR1 Antibody (DMC465) - Chimeric

CCR1 Antibody (DMC465) - Chimeric

Flow Cytometry: CCR1 Antibody (DMC465) - Chimeric [NBP3-48348]

CCR1 protein is highly expressed on the surface of HEK293 cell membrane. Flow cytometry analysis with Anti-CCR1 (DMC465) on HEK293 cells transfected with human CCR1 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram), and Isotype antibody on HEK293 transfected with irrelevant protein (Orange histogram).
CCR1 Antibody (DMC465) - Chimeric

Western Blot: CCR1 Antibody (DMC465) - Chimeric [NBP3-48348]

Anti-CCR1 antibody (Catalog Number# NBP3-48348) at 1/1000 dilutionLane : Hela, whole cell lysateSecondary : Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilutionPredicted band size: 41 kDaObserved band size: 50 kDa
CCR1 Antibody (DMC465) - Chimeric

Flow Cytometry: CCR1 Antibody (DMC465) - Chimeric [NBP3-48348]

Flow cytometry analysis of antigen binding of anti-human CCR1 mAb(NBP3-48348). (A) NBP3-48348 does not bind to CHO-S cells that do not express CCR1. (B) A clear peak shift of NBP3-48348 was seen compared to the control when incubated with CCR1-expressing 8226 cells, indicating strong binding of NBP3-48348 to CCR1. Antibodies were incubated at 5 ug/ml.

Applications for CCR1 Antibody (DMC465) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:1000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCR1

Receptors for the C-C chemokine family include CCR-1, CCR-2A, CCR-3, CCR-4, CCR-5 and the Duffy blood group antigen. The C-C receptors are important in the function of T cell chemotaxis and migration of phagocytic cells to sites of inflammation.

Alternate Names

CCR1, CD191

Gene Symbol

CCR1

Additional CCR1 Products

Product Documents for CCR1 Antibody (DMC465) - Chimeric

Certificate of Analysis

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Product Specific Notices for CCR1 Antibody (DMC465) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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