CD200/OX2 Antibody (OX-2) - BSA Free
Novus Biologicals | Catalog # NB100-65320
![Flow Cytometry: CD200/OX2 Antibody (OX-2) - BSA Free [NB100-65320]](https://beta-resources.rndsystems.com/images/products/CD200-OX2-Antibody-OX-2-Flow-Cytometry-NB100-65320-img0002.jpg "Flow Cytometry: CD200/OX2 Antibody (OX-2) - BSA Free [NB100-65320]")
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OX-2
Format
BSA Free
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Product Specifications
Immunogen
Rat thymocyte membrane glycoproteins
Localization
Membrane; single pass type I membrane protein.
Specificity
NB100-65320 recognizes the rat CD200 cell surface antigen, also known as OX-2, a type I membrane glycoprotein, expressed on rat thymocytes, neurons, follicular dendritic cells, endothelium and at low levels on some B cells and smooth muscle cells. Studies have indicated that the CD200-CD200 ligand system is important in the control of macrophage and granulocyte activation and that this antibody blocks OX-2 mediated T cell costimulation. This product is routinely tested by flow cytometry on rat thymocytes.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse CD200/OX2 Antibody (OX-2) - BSA Free (NB100-65320) is a monoclonal antibody validated for use in IHC, Flow and IP. Anti-CD200/OX2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD200/OX2 Antibody (OX-2) - BSA Free
Flow Cytometry: CD200/OX2 Antibody (OX-2) - BSA Free [NB100-65320]
Flow Cytometry: CD200/OX2 Antibody (OX-2) [NB100-65320] - Analysis of PE conjugate of NB100-65320. Staining of rat thymocytes with Mouse anti Rat CD200![Flow Cytometry: CD200/OX2 Antibody (OX-2) - BSA Free [NB100-65320]](https://beta-resources.rndsystems.com/images/products/CD200-OX2-Antibody-OX-2-Flow-Cytometry-NB100-65320-img0001.jpg "Flow Cytometry: CD200/OX2 Antibody (OX-2) - BSA Free [NB100-65320]")
Flow Cytometry: CD200/OX2 Antibody (OX-2) - BSA Free [NB100-65320]
Flow Cytometry: CD200/OX2 Antibody (OX-2) [NB100-65320] - Staining of rat thymocytes with Mouse anti Rat CD200/OX2.Applications for CD200/OX2 Antibody (OX-2) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD200
Additional CD200 Products
Product Documents for CD200/OX2 Antibody (OX-2) - BSA Free
Product Specific Notices for CD200/OX2 Antibody (OX-2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for CD200/OX2 Antibody (OX-2) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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