CD23/Fc epsilon RII Antibody (OTI2B6)

Novus Biologicals | Catalog # NBP2-45624

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2B6
View all CD23/Fc epsilon RII Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 48-321 of human FCER2 (NP_001993) produced in SF9 cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

36.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD23/Fc epsilon RII Antibody (OTI2B6)

Western Blot: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624]

Western Blot: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624]

Western Blot: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD23.
Immunohistochemistry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624]

Immunohistochemistry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624]

Immunohistochemistry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Flow Cytometry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624]

Flow Cytometry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624]

Flow Cytometry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624] - analysis of living 293T cells transfected with FCER2 overexpression plasmid.
Immunohistochemistry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624]

Immunohistochemistry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624]

Immunohistochemistry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Flow Cytometry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624]

Flow Cytometry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624]

Flow Cytometry: CD23/Fc epsilon RII Antibody (OTI2B6) [NBP2-45624] - analysis of living RPMI-8226 cells, using anti-FCER2 antibody, compared to an isotype control (green), and a PBS control (blue) (1:100).

Applications for CD23/Fc epsilon RII Antibody (OTI2B6)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD23/Fc epsilon RII

The CD23 antigen is the low affinity IgE Fc receptor, which is a 49 kDa protein with 38 and 28 kDa fragments. It is expressed on most mature, conventional B cells (but not on peritoneal CD5+ B cells), and can also be found on the surface of T cells, macrophages, platelets and EBV transformed B lymphoblasts. Expression of CD23 has been detected in neoplastic cells from cases of B cell chronic Lymphocytic leukemia. CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils. CD23 is distinct from the high affinity IgE receptors found on basophils and mast cells, which mediate allergic reactions. The low affinity receptors are thought to play a role in isotype specific immunoregulation. The regulation of CD23 surface expression appears to be integral with the complex IgE system, which involves interactions of cells, cytokines, antibodies and regulatory factors. CD23 has been described as a

Long Name

Fc epsilon Receptor II

Alternate Names

CD23, CLEC4J, Fc epsilon RII, FCER2, Fcer2a, FceRII, IGEBF, Ly-42

Gene Symbol

FCER2

Additional CD23/Fc epsilon RII Products

Product Documents for CD23/Fc epsilon RII Antibody (OTI2B6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD23/Fc epsilon RII Antibody (OTI2B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD23/Fc epsilon RII Antibody (OTI2B6)

There are currently no reviews for this product. Be the first to review CD23/Fc epsilon RII Antibody (OTI2B6) and earn rewards!

Have you used CD23/Fc epsilon RII Antibody (OTI2B6)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies