CD3 epsilon Antibody (C3e/1308) [Biotin]

Novus Biologicals | Catalog # NBP2-54392B

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Multiplex Immunoassay, CyTOF-ready

Label

Biotin

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # C3e/1308
View all CD3 epsilon Primary Antibodies »

Product Specifications

Immunogen

Recombinant human CD3 epsilon fragment (aa 23-119) (Uniprot: P07766)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 33428661).

Localization

Cell surface and Cytoplasmic

Marker

T-Cell Marker

Specificity

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for CD3 epsilon Antibody (C3e/1308) [Biotin]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Multiplex Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Multiplex Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Use in Multiplex Immunoassay reported in scientific literature (PMID: 31942075).
Optimal dilution of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 1 using NBP2-54392B in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: CD3 epsilon

CD3 epsilon is a subunit of CD3. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (C3e/1308) [Biotin]

Certificate of Analysis

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Product Specific Notices for CD3 epsilon Antibody (C3e/1308) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD3 epsilon Antibody (C3e/1308) [Biotin] (1)

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  • CD3 epsilon Antibody (C3e/1308) [Biotin]
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Spleen
    Species: Mouse
    Verified Customer | Posted 03/18/2024
    Mouse FFPE spleen section incubated with 1 to 50 dilution NBP2-54392B for 16h at 4 degrees Celsius. No specific labeling was observed in a section known to be CD3 positive.
    Target Retrieval Solutions were used as antigen retrieval solutions. Retrieval was carried out for 20m in a vegetable steamer.
    CD3 epsilon Antibody (C3e/1308) [Biotin] NBP2-54392B
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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Protocols

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