CD3 epsilon Antibody (UCHT1) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80638

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Porcine, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Radioimmunodiffusion, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # UCHT1

Format

Azide and BSA Free
View all CD3 epsilon Primary Antibodies »

Product Specifications

Immunogen

Human infant thymocytes and Sezary cells.

Epitope

Epsilon-chain

Localization

Type I membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD3 epsilon Antibody (UCHT1) - Azide and BSA Free

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638] - Staining of human peripheral blood lymphocytes with Mouse anti-Human CD3: RPE. Image from the standard format of this antibody.
Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638]

Flow Cytometry: CD3 epsilon Antibody (UCHT1) - Azide and BSA Free [NBP2-80638] - Analysis of PE/Cy5 conjugate of NB100-2726. Staining of normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control PE-Cy5 (green) or Anti-Human CD3 PE-Cy5 (blue). Cells in the lymphocyte gate were used for analysis.

Applications for CD3 epsilon Antibody (UCHT1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunoprecipitation

1:10-1:500

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD3 epsilon

CD3 complex is crucial in transducing antigen-recognition signals into the cytopasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Alternate Names

CD3e

Gene Symbol

CD3E

Additional CD3 epsilon Products

Product Documents for CD3 epsilon Antibody (UCHT1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD3 epsilon Antibody (UCHT1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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