CD3 gamma Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31250

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CD3 gamma Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human CD3 gamma. The exact sequence is proprietary.

Localization

Membrane

Specificity

This antibody is specific to the human CD3G protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD3 gamma Antibody - BSA Free

Western Blot: CD3 gamma Antibody [NBP1-31250]

Western Blot: CD3 gamma Antibody [NBP1-31250]

Western Blot: CD3 gamma Antibody [NBP1-31250] - Sample (30 ug of whole cell lysate) A: Molt-4 12% SDS PAGE, antibody diluted at 1:3000.
Immunohistochemistry-Paraffin: CD3 gamma Antibody [NBP1-31250]

Immunohistochemistry-Paraffin: CD3 gamma Antibody [NBP1-31250]

Immunohistochemistry-Paraffin: CD3 gamma Antibody [NBP1-31250] - Human PANC1 xenograft. CD3 gamma antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Flow Cytometry: CD3 gamma Antibody [NBP1-31250]

Flow Cytometry: CD3 gamma Antibody [NBP1-31250]

Flow Cytometry: CD3 gamma Antibody [NBP1-31250] - Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4 degrees C for 15 min. Black: Unlabelled sample was used as a control. Red: CD3 gamma antibody dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

Applications for CD3 gamma Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CD3 gamma

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.

Alternate Names

CD3g, IMD17, T3G

Gene Symbol

CD3G

Additional CD3 gamma Products

Product Documents for CD3 gamma Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD3 gamma Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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