CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-11991

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Block/Neutralize, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Functional Assay

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # RM153

Format

Azide and BSA Free
View all CD30 Ligand/TNFSF8 Primary Antibodies »

Product Specifications

Immunogen

This antibody was raised by immunising Sprague Dawley rats with the CHO cells stably transfected with murine CD30 Ligand/TNFSF8 and fusing the splenocytes with P3U1 myeloma cells.

Specificity

This antibody is specific for murine CD30 Ligand/TNFSF8, a 40-kDa type II transmembrane protein member of the TNF superfamily. Interaction of CD30 with CD30 Ligand/TNFSF8 plays an important role in T cell activation/costimulation.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free [NBP3-11991]

Immunocytochemistry/ Immunofluorescence: CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free [NBP3-11991]

Immunocytochemistry/Immunofluorescence: CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric [NBP3-11991] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RG9-35 (NBP3-11991) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11991, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.

Applications for CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Block/Neutralize

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Functional Assay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD30 Ligand/TNFSF8

CD153 is a 39-40 kD type II membrane glycoprotein, also known as CD30 ligand or CD30L. This TNF superfamily member is expressed on activated T cells. CD153 has been reported to stimulate B-cell proliferation, antigen-specific antibody production, polyclonal immunoglobulin secretion, and T cell activation and proliferation. The RM153 antibody inhibits binding of CD153 to CD30.

Alternate Names

CD153, CD30L, TNFSF8

Gene Symbol

TNFSF8

Additional CD30 Ligand/TNFSF8 Products

Product Documents for CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free

There are currently no reviews for this product. Be the first to review CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free and earn rewards!

Have you used CD30 Ligand/TNFSF8 Antibody (RM153) - Chimeric - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies