CD30/TNFRSF8 Antibody (Ber-H8) - BSA Free

Novus Biologicals | Catalog # NBP3-09139

Clone Ber-H8 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Ber-H8

Format

BSA Free
View all CD30/TNFRSF8 Primary Antibodies »

Product Specifications

Immunogen

Immunogen from CD30/TNFRSF8

Specificity

The mouse monoclonal antibody Ber-H8 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD30/TNFRSF8 Antibody (Ber-H8) - BSA Free

Flow Cytometry: CD30/TNFRSF8 Antibody (Ber-H8) - BSA Free [NBP3-09139]

Flow Cytometry: CD30/TNFRSF8 Antibody (Ber-H8) - BSA Free [NBP3-09139]

Flow Cytometry: CD30/TNFRSF8 Antibody (Ber-H8) [NBP3-09139] - Analysis (surface staining) of K562 cells with anti-human CD30 (Ber-H8) purified, GAM APC.

Applications for CD30/TNFRSF8 Antibody (Ber-H8) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry-Frozen

5-10 ug/ml

Immunohistochemistry-Paraffin

5-10 ug/ml

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD30/TNFRSF8

CD30 is a type I transmembrane protein and a member of the tumor necrosis factor receptor superfamily of proteins. (1,2) Murine CD30 is expressed predominantly in the thymus, and it is inducible in mouse splenocytes stimulated with pokeweed mitogen or concanavalin A. (1) In anti-CD3epsilon-activated spleen cells, CD30 is expressed primarily on the surface of CD8+ T cells with peak expression on days 4 and 5. Stimulation of CD30+ CTL lines with plate-bound anti-CD30 directly signals IL-5 but not IFN; production. (1) While these studies demonstrate that CD30 directs cytokine secretion and suggest that CD30 may play a pivotal role in the pattern of cytokine production by T cells, the precise roles of CD30 and its ligand (CD153) in T-cell development have not been clearly defined.

Alternate Names

CD30, TNFRSF8

Gene Symbol

TNFRSF8

Additional CD30/TNFRSF8 Products

Product Documents for CD30/TNFRSF8 Antibody (Ber-H8) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD30/TNFRSF8 Antibody (Ber-H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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