CD35 Antibody (8C12) - Chimeric - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-52668

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # 8C12

Format

Azide and BSA Free
View all CD35 Primary Antibodies »

Product Specifications

Immunogen

Purified murine CR1 protein.

Specificity

Recognises an epitope on CD35 (CR1, 190 kDa), but not on CD21 (CR2, 145-150 kDa), which are alternative splice variants of primary Cr2 gene transcripts.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for CD35 Antibody (8C12) - Chimeric - Azide and BSA Free

Immunohistochemistry: CD35 Antibody (8C12) - Chimeric - Azide and BSA Free [NBP2-52668]

Immunohistochemistry: CD35 Antibody (8C12) - Chimeric - Azide and BSA Free [NBP2-52668]

Immunohistochemistry: CD35 Antibody (8C12) - Chimeric [NBP2-52668] - Immunohistochemical staining of rat stomach using anti-Complement receptor 1 antibody NBP2-52668. Formalin fixed rat stomach slices were were stained with NBP2-52668 at 3 ug/ml.

Applications for CD35 Antibody (8C12) - Chimeric - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques. This antibody recognises murine CD35, expressed on the majority of peripheral B-cells and peritoneal macrophages and upon fMet-Leu-Phe stimulation.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: CD35

CD35 encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.

Alternate Names

C3BR, CD35, CR1, KN

Gene Symbol

CR1

Additional CD35 Products

Product Documents for CD35 Antibody (8C12) - Chimeric - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD35 Antibody (8C12) - Chimeric - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

B Cell Activation
Complement Pathway

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Protocols

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