CD39L4/ENTPD5 Antibody (064) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-89830
Recombinant Monoclonal Antibody
![Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830]](https://resources.rndsystems.com/images/products/CD39L4-ENTPD5-Antibody-064-Western-Blot-NBP2-89830-img0001.jpg "Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 064 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD39L4/ENTPD5 (Uniprot#: O75356; Met1-His428).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for CD39L4/ENTPD5 Antibody (064) - Azide and BSA Free
Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830]
Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Anti-CD39L4/ENTPD5 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 53 kDa
Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] -
Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human liver with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] -
Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human kidney with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] -
Immunoprecipitation: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - ENTPD5 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 0.5 uL NBP2-89830 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP2-89830 at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 47 kDaApplications for CD39L4/ENTPD5 Antibody (064) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:500-1:2000
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Application Notes
This antibody will detect Human CD39L4/ENTPD5 in ELISA pair set (Catalog: # NBP2-79350). In a sandwich ELISA, it can be used as a capture antibody when paired with (Catalog: # NBP2-89829).
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD39L4/ENTPD5
Long Name
Ecto-Nucleoside Triphosphate Diphosphohydrolase 5
Alternate Names
CD39L4, ENTPD5, ER-UDPase, NTPDase 5, PCPH
Gene Symbol
ENTPD5
Additional CD39L4/ENTPD5 Products
Product Documents for CD39L4/ENTPD5 Antibody (064) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD39L4/ENTPD5 Antibody (064) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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