CD40 Ligand/TNFSF5 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-16982
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EMQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQL
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CD40 Ligand/TNFSF5 Antibody - BSA Free (NBP3-16982) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CD40 Ligand/TNFSF5 Antibody - BSA Free
Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP3-16982]
Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP3-16982] - Staining of human lymph node shows high expression.Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP3-16982]
Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP3-16982] - Staining of human cerebral cortex shows low expression as expected.Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP3-16982]
Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP3-16982] - Analysis in human lymph node and cerebral cortex tissues using Anti-CD40LG antibody. Corresponding CD40LG RNA-seq data are presented for the same tissues.Applications for CD40 Ligand/TNFSF5 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:2500 - 1:5000
Immunohistochemistry-Paraffin
1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD40 Ligand/TNFSF5
Alternate Names
CD154, CD40L, CD40LG, gp39, TNFSF5, TRAP
Gene Symbol
CD40LG
Additional CD40 Ligand/TNFSF5 Products
- All Products for CD40 Ligand/TNFSF5
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- CD40 Ligand/TNFSF5 ELISA Kits
- CD40 Ligand/TNFSF5 Luminex Assays
- CD40 Ligand/TNFSF5 Lysates
- CD40 Ligand/TNFSF5 Primary Antibodies
- CD40 Ligand/TNFSF5 Proteins and Enzymes
- CD40 Ligand/TNFSF5 Simple Plex
- CD40 Ligand/TNFSF5 Small Molecules and Peptides
Product Documents for CD40 Ligand/TNFSF5 Antibody - BSA Free
Product Specific Notices for CD40 Ligand/TNFSF5 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for CD40 Ligand/TNFSF5 Antibody - BSA Free
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Q: I am looking for paired ABs for use in detecting CD40L (rat) by ELISA. Do you have such a reagent(s) available?
A: Unfortunately we currently do not have any CD40 Ligand antibody pairs that have been validated in Rat. The only antibody pairs we offer for CD40L are validated in Human. I apologize for this inconvenience. If you would be interested in testing one of our products in Rat I'd like to invite you to participate in our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
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