CD42b/GPIb alpha Antibody (HIP1) - BSA Free

Novus Biologicals | Catalog # NB500-511

Clone HIP1 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, CyTOF-ready

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # HIP1

Format

BSA Free
View all CD42b/GPIb alpha Primary Antibodies »

Product Specifications

Immunogen

Peripheral blood mononuclear cells of a patient suffering with CLL.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31467123).

Specificity

The antibody HIP1 reacts with CD42b (GPIb alpha), a 135-145 kDa membrane glycoprotein expressed on platelets and megakaryocytes. CD42b and CD42c (GPIb beta) are composed in a disulfide linked heterodimer (CD42b/c; 160 kDa); CD42b/c forms a noncovalent complex with CD42a and CD42d. HLDA IV; WS Code P 40

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

145 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD42b/GPIb alpha Antibody (HIP1) - BSA Free

Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) - BSA Free [NB500-511]

Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) - BSA Free [NB500-511]

Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) [NB500-511] - Staining of human peripheral blood with anti-CD42b (HIP1) APC.
Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) - BSA Free [NB500-511]

Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) - BSA Free [NB500-511]

Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) [NB500-511] - Staining of human platelets with anti-CD42b (HIP1) PE.
Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) - BSA Free [NB500-511]

Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) - BSA Free [NB500-511]

Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) [NB500-511] - Staining of human peripheral blood with anti-CD42b (HIP1) purified / GAM-APC.
CD42b/GPIb alpha Antibody (HIP1) - BSA Free Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) - BSA Free [NB500-511]

Flow Cytometry: CD42b/GPIb alpha Antibody (HIP1) - BSA Free [NB500-511]

Surface staining pattern of human peripheral blood cells stained using anti-human CD42b (HIP1) purified antibody (concentration in sample 4 ug/ml) GAM APC.

Applications for CD42b/GPIb alpha Antibody (HIP1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-4 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:200
Application Notes
Functional Application: The antibody HIP1 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination. This antibody is CyTOF ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD42b/GPIb alpha

CD42b (GPIb alpha) composes together with GPIb beta, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.

Long Name

Glycoprotein lb [Platelet] alpha

Alternate Names

BP1BA, BSS, CD42b, GP1BA, GPIb alpha

Entrez Gene IDs

2811 (Human)

Gene Symbol

GP1BA

Additional CD42b/GPIb alpha Products

Product Documents for CD42b/GPIb alpha Antibody (HIP1) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD42b/GPIb alpha Antibody (HIP1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD42b/GPIb alpha Antibody (HIP1) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CD42b/GPIb alpha Antibody (HIP1) - BSA Free

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  • Q: I want to buy the anti- CD42b/GPIbA monclonal antibody, which is used to inject into the mouse to make the animal model of thrombopenia. Can the anti- CD42b/GPIbA antibody(NB500-511) be used in this experiment?

    A: NB500-511 inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination. I am not familiar enough with your thrombopenia model to say for certain if this antibody will work for you studies, but this should give you some insight as to whether or not it is suitable for your studies. Furthermore, this antibody does contain 15mM sodium azide which would have to be removed before injection. We have kits that will allow you to do this clean-up, if you are interested.

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