CD44 Antibody (VFF-8) - variant 5 - BSA Free
Novus Biologicals | Catalog # NB100-65533
![Immunohistochemistry-Paraffin: CD44 Antibody (VFF-8) - variant 5 [NB100-65533]](https://resources.rndsystems.com/images/products/CD44-variant-5-Antibody-VFF-8-Immunohistochemistry-Paraffin-NB100-65533-img0002.jpg "Immunohistochemistry-Paraffin: CD44 Antibody (VFF-8) - variant 5 [NB100-65533]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # VFF-8
Format
BSA Free
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Product Specifications
Immunogen
Made to Human CD44v5
Marker
Cell Membrane Marker
Specificity
Specifically recognizes an epitope encoded by exon v5 on the variant portion of human CD44.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for CD44 Antibody (VFF-8) - variant 5 - BSA Free
Immunohistochemistry-Paraffin: CD44 Antibody (VFF-8) - variant 5 [NB100-65533]
Immunohistochemistry-Paraffin: CD44, variant 5 Antibody (VFF-8) [NB100-65533] - Human breast cancer tissue with Mouse anti Human CD44v5 antibody.![Immunohistochemistry-Paraffin: CD44 Antibody (VFF-8) - variant 5 [NB100-65533]](https://resources.rndsystems.com/images/products/CD44-variant-5-Antibody-VFF-8-Immunohistochemistry-Paraffin-NB100-65533-img0001.jpg "Immunohistochemistry-Paraffin: CD44 Antibody (VFF-8) - variant 5 [NB100-65533]")
Immunohistochemistry-Paraffin: CD44 Antibody (VFF-8) - variant 5 [NB100-65533]
Immunohistochemistry-Paraffin: CD44, variant 5 Antibody (VFF-8) [NB100-65533] - Human breast cancer tissue with Mouse anti Human CD44v5 antibody.Applications for CD44 Antibody (VFF-8) - variant 5 - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10
Immunohistochemistry-Paraffin
1:10
Western Blot
1:100-1:2000
Application Notes
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium Citrate buffer pH6.0 is recommended for this purpose.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS
Format
BSA Free
Preservative
0.1% Sodium Azide
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CD44
Alternate Names
CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1
Entrez Gene IDs
960 (Human)
Gene Symbol
CD44
UniProt
Additional CD44 Products
Product Documents for CD44 Antibody (VFF-8) - variant 5 - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CD44 Antibody (VFF-8) - variant 5 - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Hematopoietic Stem Cell Differentiation Pathways & Lineage-specific Markers
Innate Lymphoid Cell Differentiation Pathways
Mesenchymal Stem Cell Differentiation Pathways & Lineage-specific Markers
Neural Stem Cell Differentiation Pathways & Lineage-specific Markers
