CD46 Antibody (M037)

Novus Biologicals | Catalog # NBP3-23191

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # M037
View all CD46 Primary Antibodies »

Product Specifications

Immunogen

Clone (M037) was generated from a proprietary antigen related to native human CD46 expressed in NCI-H1915 lung cancer cell line. [UniProt# P15529]

Specificity

Clone M037 mouse monoclonal antibody detects a 45-60 kDa protein on SDS-PAGE "Native" immunoblots of human A549, MeWo, MDA-MB-231, and MCF7 cells, as well as human lung tissues. This antibody weakly detects denatured CD46.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for CD46 Antibody (M037)

CD46 Antibody (M037)

Immunocytochemistry/Immunofluorescence: CD46 Antibody (M037) [NBP3-23191] -

Immunocytochemistry/Immunofluorescence: CD46 Antibody (M037) [NBP3-23191] - Immunocytochemical labeling of CD46 in aldehyde fixed human NCIH1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD46 (NBP3-23191). The antibody was detected using goat anti-mouse Ig:DyLight(R) 594.
CD46 Antibody (M037)

Immunocytochemistry/Immunofluorescence: CD46 Antibody (M037) [NBP3-23191] -

Immunocytochemistry/Immunofluorescence: CD46 Antibody (M037) [NBP3-23191] - Immunocytochemical labeling of CD46 in aldehyde fixed human NCIH1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD46 (NBP3-23191). The antibody was detected using goat anti-mouse Ig:DyLight(R) 594.
CD46 Antibody (M037)

Western Blot: CD46 Antibody (M037) [NBP3-23191] -

Western Blot: CD46 Antibody (M037) [NBP3-23191] - Western blot of native lysates from NCI-H2052 mesothelial cells (lane 1), SK-MES-1 squamous carcinoma (lane 2), MDA-MB-231 breast carcinoma (lane 3), MeWo melanoma (lane 4), A431 skin adenocarcinoma (lane 5), LNCaP prostate cancer cells (lane 6), and MCF7 breast cancer cells (lane 7). The blot was probed with mouse monoclonal anti-CD46 (NBP3-23191) at 1:1000.

Applications for CD46 Antibody (M037)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunoprecipitation

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: CD46

CD46 is one of the membrane complement regulatory proteins. CD46 possesses C3b-binding and factor I cofactor activities which play important roles in regulation of complement activation pathway. CD46 is widely distributed on blood cells, endothelial cells, epithelial cells and tumor cell lines. CD46 exists as many isoforms in a variety of tissues. The antigen has a broad distribution and is present on leukocytes, platelets, endothelial cells, epithelial cells and fibroblasts. It is strongly expressed on salivary gland ducts and kidney ducts, moderately on lymphocytes and endothelium, and weakly on interstitial tissues and muscle cells, but not on erythrocytes. It is expressed on thymocytes, B cells, monocytes, granulocytes, NK cells, platelets, epithelial and endothelial cells.

Alternate Names

CD46, MCP

Gene Symbol

CD46

Additional CD46 Products

Product Documents for CD46 Antibody (M037)

Certificate of Analysis

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Download SDS

Product Specific Notices for CD46 Antibody (M037)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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