CD55/DAF Antibody (028) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89269

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA Capture, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 028 expressed in HEK293

Format

Azide and BSA Free
View all CD55/DAF Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD55/DAF (Accession#: NP_000565.1; Met 1-Ser 353).

Specificity

No cross-reactivity in ELISA with: Human CD97

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD55/DAF Antibody (028) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CD55/DAF Antibody (028) [NBP2-89269]

Immunocytochemistry/ Immunofluorescence: CD55/DAF Antibody (028) [NBP2-89269]

Immunocytochemistry/Immunofluorescence: CD55/DAF Antibody (028) [NBP2-89269] - Staining of Human CD55 in JURKAT cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with Rabbit anti-Human CD55 monoclonal antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Flow Cytometry: CD55/DAF Antibody (028) [NBP2-89269]

Flow Cytometry: CD55/DAF Antibody (028) [NBP2-89269]

Flow Cytometry: CD55/DAF Antibody (028) [NBP2-89269] - Analysis of human CD55 expression on K562 cells. Cells were stained with purified anti-Human CD55, then a FITC-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.

Applications for CD55/DAF Antibody (028) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Flow Cytometry

1:25-1:100

Immunocytochemistry/ Immunofluorescence

1:10-1:40

Sandwich ELISA Capture

1:250-1:2000
Application Notes
This antibody will detect Human CD55/DAF in ELISA pair set (Catalog: # NBP2-79440).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD55/DAF

CD55 encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Alternate Names

CD55, CR, DAF, TC

Gene Symbol

CD55

Additional CD55/DAF Products

Product Documents for CD55/DAF Antibody (028) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CD55/DAF Antibody (028) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Complement Pathway

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Protocols

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FAQs

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