CD55/DAF Antibody (MEM-118) - BSA Free

Novus Biologicals | Catalog # NB500-395

Clone MEM-118 was used by HLDA to establish CD designation.
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Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # MEM-118

Format

BSA Free
View all CD55/DAF Primary Antibodies »

Product Specifications

Immunogen

HPB-ALL human T cell line

Specificity

The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells; it is weakly present on NK cells. HLDA V; WS Code AS S016

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD55/DAF Antibody (MEM-118) - BSA Free

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) purified, GAM-APC.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood leukocytes by mouse monoclonal anti-CD55 antibody MEM-118.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) PE-Cy(TM)5.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) PE.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) Alexa Fluor 488.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) biotin / streptavidin-APC.
Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395]

Flow (Cell Surface): CD55/DAF Antibody (MEM-118) [NB500-395] - Surface staining of human peripheral blood cells with anti-CD55 (MEM-118) Alexa Fluor 647.

Applications for CD55/DAF Antibody (MEM-118) - BSA Free

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Multi-step

Formulation

Tris buffered saline (TBS), pH 8.0

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD55/DAF

Avoidance by host tissues of attack by autologous complement proteins is dependent in part on the activities of membrane regulatory factors. One molecule involved in this control is a 70 kD glycoprotein termed decay-accelerating factor (DAF), or CD55. Interruption by CD55 of the complement sequence at an early step in activation effectively halts progression of the cascade and prevents consequent cell injury. In man, CD55 is expressed on the plasma membrane of all cell types that are in intimate contact with plasma complement proteins. This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. CD55 also acts as the receptor for echovirus 7 and related viruses(echoviruses 13, 21, 29 and 33). It is attached to the membrane by a GPI-anchor.

Alternate Names

CD55, CR, DAF, TC

Entrez Gene IDs

1604 (Human)

Gene Symbol

CD55

Additional CD55/DAF Products

Product Documents for CD55/DAF Antibody (MEM-118) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD55/DAF Antibody (MEM-118) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Complement Pathway

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Protocols

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