CD57 Antibody (IHC539)

Novus Biologicals | Catalog # NBP2-75981

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Kappa Clone # IHC539
View all CD57 Primary Antibodies »

Product Specifications

Immunogen

Recombinant Human CD57

Clonality

Monoclonal

Host

Mouse

Isotype

IgM Kappa

Theoretical MW

110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD57 Antibody (IHC539)

Immunohistochemistry-Paraffin: CD57 Antibody (IHC539) [NBP2-75981]

Immunohistochemistry-Paraffin: CD57 Antibody (IHC539) [NBP2-75981]

Immunohistochemistry-Paraffin: CD57 Antibody (IHC539) [NBP2-75981] - Staining using CD57 antibody (IHC539) in tonsil

Applications for CD57 Antibody (IHC539)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:200

Immunohistochemistry-Paraffin

1:100 - 1:200
Application Notes
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. Positive control: Tonsil. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Formulation, Preparation, and Storage

Purification

Protein L purified

Formulation

Tris Buffer, pH 7.3 - 7.7, with 1% BSA

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: CD57

The CD57 antigen, also referred to as human natural killer-1 (HNK-1) and LEU-7, is a carbohydrate epitope containing sulfated glucuronic acid that is expressed by many cell types within the nervous system (1,2). Cellular expression of CD57 changes during progression of neural development (1). The CD57 epitope is synthesized by enzymes including a sulfotransferase and one of two glucotransferases (GlcAT-P or GlcAT-S) (1,2). The structure of the CD57/HNK-1 epitope consists of 3-O-sulfated glucuronic acid (GlcA) attached to a terminal galactose (HSO3-3GlcAbeta1-3Galbeta1-4GlcNAc-R) (1,2). The CD57 epitope was first identified and a marker for HNK cells and its expression is associated with NK cell maturation (1-3).

CD57 expression has become a common pathological marker. CD57+ CD8+ T cell expression is observed in various cancers and is often linked to reduced survival (3). On the other hand, CD57+ NK cell expression is associated with less severe disease and better prognosis in cancer patients (3). CD57 is also associated with immune dysfunction and autoimmune disease (3,4). For example, T cell immunosenescence is characterized by an upregulation of CD57 along with KLRG-1 and a loss of CD27 and CD28 expression (4). Expanded autoreactive T cells are also related to diseases including multiple sclerosis (MS) and rheumatoid arthritis (RA) (3). Conversely, reduced numbers of CD57+ NK cells and NK cell cytotoxicity is observed in various autoimmune diseases such as apoptotic dermatitis and sjogren's syndrome (3).

References

1. Focosi D, Bestagno M, Burrone O, Petrini M. CD57+ T lymphocytes and functional immune deficiency. J Leukoc Biol. 2010;87(1):107-116. https://doi.org/10.1189/jlb.0809566

2. Morise J, Takematsu H, Oka S. The role of human natural killer-1 (HNK-1) carbohydrate in neuronal plasticity and disease. Biochim Biophys Acta Gen Subj. 2017;1861(10):2455-2461. https://doi.org/10.1016/j.bbagen.2017.06.025

3. Nielsen CM, White MJ, Goodier MR, Riley EM. Functional Significance of CD57 Expression on Human NK Cells and Relevance to Disease. Front Immunol. 2013;4:422. Published 2013 Dec 9. https://doi.org/10.3389/fimmu.2013.00422

4. Lian J, Yue Y, Yu W, Zhang Y. Immunosenescence: a key player in cancer development. J Hematol Oncol. 2020;13(1):151. Published 2020 Nov 10. https://doi.org/10.1186/s13045-020-00986-z6

Long Name

Cluster of Differentiation 57

Alternate Names

Human Natural Killer-1, L2, LEU-7

Gene Symbol

B3GAT1

Additional CD57 Products

Product Documents for CD57 Antibody (IHC539)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CD57 Antibody (IHC539)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for CD57 Antibody (IHC539) (NBP2-75981):

Immunohistochemistry Protocol


Specimen Collection and Preparation for Analysis

Each tissue section should be fixed with 10% neutral buffered formalin, cut to the applicable thickness (4um), and placed on a glass slide that is positively charged. The prepared slide may then be baked for a minimum of 30 minutes in a 53-65 degrees C oven (do not exceed 24 hours).


Recommended Staining Protocols

Manual Use:
1. Pretreatment: Perform heat-induced epitope retrieval (HIER) at pH 9 for 10 to 30 minutes.
2. Peroxide Block: Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System.)
3. Primary Antibody: Apply antibody directly (Predilute) or dilute antibody at 1:100-1:200 (Concentrate) before applying. Incubate antibody for 10 to 30 minutes at room temperature.
4. Secondary Antibody: Incubate for 20 to 30 minutes at room temperature.
5. Substrate Development: Incubate DAB or Fast Red for 5 to 10 minutes at room temperature.
6. Counterstain: Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds.
7. Dehydrate and apply coverslip.

Automated Staining System:
The stated primary antibody has been optimized and validated using the BOND-MAX fully automated IHC & ISH stainer manufactured by Leica Biosystems, applying IHC Protocol F. The following edits are recommended for the protocol:
a) Marker Incubation Time: 30 minutes
b) Heat-induced epitope retrieval (HIER) is recommended using Leica Bond ER Solution 2 for 30 minutes.
c) Move Peroxide Block step to after Polymer and before Mixed DAB Refine. For all other automated IHC staining systems, refer to the corresponding user manual for specific instructions.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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