CD59 Antibody (MEM-43) - BSA Free

Novus Biologicals | Catalog # NB500-330

Clone MEM-43 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Block/Neutralize, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Cited:

Block/Neutralize

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-43

Format

BSA Free
View all CD59 Primary Antibodies »

Product Specifications

Immunogen

Thymocytes and T lymphocytes (NM_000611.5).

Specificity

The antibody MEM-43 reacts with well defined epitope (W40, R-53) on CD59 (Protectin), an 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells; it is widely present on cells in all tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD59 Antibody (MEM-43) - BSA Free

Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Immunohistochemistry-Paraffin: CD59 Antibody (MEM-43) [NB500-330] - Staining of human placenta (paraffin sections) using anti-CD59 (MEM-43).
Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) Pacific BlueTM.
Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Immunohistochemistry-Frozen: CD59 Antibody (MEM-43) [NB500-330] - Human tonsil frozen tissue section stained for CD59 (red) and counterstained with DAPI (blue). Image from verified customer review.
Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Analysis using the PE conjugate of NB500-330. Surface staining of human peripheral blood cells with anti-human CD59 (MEM-43) PE.
Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the PE direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) PE.
Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Using the Allophycocyanin direct conjugate Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) APC.
Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) FITC.
Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) [NB500-330] - Surface staining of HL-60 (positive) and SP2 (negative) cells with anti-human CD59 (MEM-43) biotin / streptavidin-APC.
CD59 Antibody (MEM-43) - BSA Free Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Flow Cytometry: CD59 Antibody (MEM-43) - BSA Free [NB500-330]

Surface staining pattern of human peripheral blood stained using anti-human CD59 (MEM-43) purified antibody (concentration in sample 0.3 ug/ml, GAM APC).

Applications for CD59 Antibody (MEM-43) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-4 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

1:50
Application Notes
Use in blocking / neutralizing reported in scientific literature (PMID 24909955). CD59 antibody validated for IHC-F from a verified customer review. This antibody is CyTOF ready.

Reviewed Applications

Read 1 review rated 4 using NB500-330 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: CD59

CD59, an LY6 like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells.

Alternate Names

CD59, HRF20, MACIF, MIC11, Protectin

Gene Symbol

CD59

Additional CD59 Products

Product Documents for CD59 Antibody (MEM-43) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD59 Antibody (MEM-43) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD59 Antibody (MEM-43) - BSA Free

Customer Reviews for CD59 Antibody (MEM-43) - BSA Free (1)

4 out of 5
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Customer Images

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  • CD59 Antibody (MEM-43)
    Name: Jan Martinek
    Application: Immunofluorescence-Frozen
    Sample Tested: Human Tonsil tissue
    Species: Human
    Verified Customer | Posted 11/15/2018
    Human tonsil stained for CD59 (red) and counterstained with DAPI (blue)
    Acetone fixation
    CD59 Antibody (MEM-43) - BSA Free NB500-330

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