CD6 Antibody (3F7B5)
Novus Biologicals | Catalog # NBP2-32942
Clone 3F7B5 was used by HLDA to establish CD designation.
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 3F7B5
Loading...
Product Specifications
Immunogen
Human rheumatoid synovial T cell line ST-1
Localization
Cell surface and cytoplasmic
Marker
Negative Marker of T-regulatory Cells
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34537)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for CD6 Antibody (3F7B5)
![Immunocytochemistry/ Immunofluorescence: CD6 Antibody (3F7B5) [NBP2-32942]](https://resources.rndsystems.com/images/products/CD6-Antibody-3F7B5-Immunocytochemistry-Immunofluorescence-NBP2-32942-img0004.jpg "Immunocytochemistry/ Immunofluorescence: CD6 Antibody (3F7B5) [NBP2-32942]")
Immunocytochemistry/ Immunofluorescence: CD6 Antibody (3F7B5) [NBP2-32942]
Immunocytochemistry/Immunofluorescence: CD6 Antibody (3F7B5) [NBP2-32942] - Immunofluorescence staining of MOLT-4 cells using CD6 Antibody (3F7B5) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.![Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942]](https://resources.rndsystems.com/images/products/CD6-Antibody-3F7B5-Immunohistochemistry-Paraffin-NBP2-32942-img0002.jpg "Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942]")
Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942]
Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942] - Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD6 MAb (3F7B5).![Flow Cytometry: CD6 Antibody (3F7B5) [NBP2-32942]](https://resources.rndsystems.com/images/products/CD6-Antibody-3F7B5-Flow-Cytometry-NBP2-32942-img0003.jpg "Flow Cytometry: CD6 Antibody (3F7B5) [NBP2-32942]")
Flow Cytometry: CD6 Antibody (3F7B5) [NBP2-32942]
Flow Cytometry: CD6 Antibody (3F7B5) [NBP2-32942] - Flow Cytometric Analysis of MOLT-4 cells. CD6 Antibody (3F7B5) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).![Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942]](https://resources.rndsystems.com/images/products/CD6-Antibody-3F7B5-Immunohistochemistry-Paraffin-NBP2-32942-img0001.jpg "Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942]")
Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942]
Immunohistochemistry-Paraffin: CD6 Antibody (3F7B5) [NBP2-32942] - Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD6 MAb (3F7B5).Applications for CD6 Antibody (3F7B5)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CD6
Additional CD6 Products
Product Documents for CD6 Antibody (3F7B5)
Product Specific Notices for CD6 Antibody (3F7B5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for CD6 Antibody (3F7B5)
There are currently no reviews for this product. Be the first to review CD6 Antibody (3F7B5) and earn rewards!
Have you used CD6 Antibody (3F7B5)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...