CD63 Antibody (OTI5E5)

Novus Biologicals | Catalog # NBP2-46167

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5E5
View all CD63 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CD63 (NP_001771) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CD63 Antibody (OTI5E5) (NBP2-46167) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD63 Antibody (OTI5E5)

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167] - CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167] - CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: CD63 Antibody (OTI5E5) [NBP2-46167]

Western Blot: CD63 Antibody (OTI5E5) [NBP2-46167]

Western Blot: CD63 Antibody (5E5) [NBP2-46167] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD63.
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human tonsil within the normal limits using CD63 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human liver tissue.. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Carcinoma of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (OTI5E5) [NBP2-46167]

Immunohistochemistry: CD63 Antibody (5E5) [NBP2-46167] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
CD63 Antibody (OTI5E5)

CD63 Antibody (OTI5E5)[NBP2-46167]

CD63 Antibody (OTI5E5)[NBP2-46167]Simple Western� analysis of endogenous protein OTC from HELA lysates (0.2 mg/mL) using OTC Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at a 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Applications for CD63 Antibody (OTI5E5)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD63

CD63 (cluster of differentiation 63), also known as lysosome associated membrane protein 3 (LAMP-3), is a membrane spanning glycoprotein with a molecular mass ranging from 30-60 kDa that was the first characterized member of the tetraspanin superfamily (1,2). CD63 is ubiquitously expressed and largely present on the cell surface in the endosomal system (1-3). More specifically, it is generally present in multivesicular bodies (MVBs), also called late endosomes, and lysosomes (1). The CD63 molecule is a total of 238 amino acids (aa), has four hydrophobic membrane-spanning regions and three N-glycosylation sites, and the encoded protein has a theoretical molecular weight of 25 kDa (2,3). CD63 both directly and indirectly interacts with other proteins including integrins, cell surface receptors, other tetraspanins, kinases, and adapter proteins, to name a few (1). CD63 is involved in many cell processes including cell survival and activation, cell adhesion, invasion, and migration (1). Additionally, CD63 has been shown to interact with tissue inhibitor of metalloproteinase 1 (TIMP-1), which originally thought to be an inhibitor of cancer progression has recently been shown to have cancer promoting properties as well (1,4). The CD63-TIMP-1 interaction has been shown to activate the PI3K/AKT pathway in lung adenocarcinoma cells and also promote survival and invasion of acute myeloid leukemia cells (1).CD63 is a useful flow cytometry marker for basophil granulocytes and can be used for the basophil activation test (BAT) to assess IgE-mediated allergy response (5). As CD63 was initially discovered on activated blood platelets and is a lysosomal-associated protein it makes sense it would be involved in platelet or lysosomal-related disorders (1,2). More precisely, CD63 is associated with Hermansky-Pudlak syndrome, a disease characterized by albinism and platelet storage pool deficiency (6).

References

1. Pols, M. S., & Klumperman, J. (2009). Trafficking and function of the tetraspanin CD63. Experimental cell research. https://doi.org/10.1016/j.yexcr.2008.09.020

2. Metzelaar, M. J., Wijngaard, P. L., Peters, P. J., Sixma, J. J., Nieuwenhuis, H. K., & Clevers, H. C. (1991). CD63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in eukaryotic cells. The Journal of biological chemistry.

3. Horejsi, V., & Vlcek, C. (1991). Novel structurally distinct family of leucocyte surface glycoproteins including CD9, CD37, CD53 and CD63. FEBS letters. https://doi.org/10.1016/0014-5793(91)80988-f

4. Eckfeld, C., HauBler, D., Schoeps, B., Hermann, C. D., & Kruger, A. (2019). Functional disparities within the TIMP family in cancer: hints from molecular divergence. Cancer metastasis reviews. https://doi.org/10.1007/s10555-019-09812-6

5. Hoffmann, H. J., Santos, A. F., Mayorga, C., Nopp, A., Eberlein, B., Ferrer, M., Rouzaire, P., Ebo, D. G., Sabato, V., Sanz, M. L., Pecaric-Petkovic, T., Patil, S. U., Hausmann, O. V., Shreffler, W. G., Korosec, P., & Knol, E. F. (2015). The clinical utility of basophil activation testing in diagnosis and monitoring of allergic disease. Allergy. https://doi.org/10.1111/all.12698

6. Dell'Angelica, E. C., Shotelersuk, V., Aguilar, R. C., Gahl, W. A., & Bonifacino, J. S. (1999). Altered trafficking of lysosomal proteins in Hermansky-Pudlak syndrome due to mutations in the beta 3A subunit of the AP-3 adaptor. Molecular cell. https://doi.org/10.1016/s1097-2765(00)80170-7

Alternate Names

CD63, Granulophysin, Lamp-3, ME491, OMA81H, Tspan30

Gene Symbol

CD63

Additional CD63 Products

Product Documents for CD63 Antibody (OTI5E5)

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Product Specific Notices for CD63 Antibody (OTI5E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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