Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 307 expressed in HEK293

Format

Azide and BSA Free
View all CD7 Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human CD7 (Accession#: NP_006128.1; Met1-Pro180).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for CD7 Antibody (307) - Azide and BSA Free

Western Blot: CD7 Antibody (307) [NBP2-89749]

Western Blot: CD7 Antibody (307) [NBP2-89749]

Western Blot: CD7 Antibody (307) [NBP2-89749] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:25 kDa Observed band size:37 kDa
Immunoprecipitation: CD7 Antibody (307) [NBP2-89749]

Immunoprecipitation: CD7 Antibody (307) [NBP2-89749]

Immunoprecipitation: CD7 Antibody (307) [NBP2-89749] - Lane A:0.5 mg Jurkat Whole Cell Lysate 2 uL anti-CD7 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CD7 rabbit monoclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 34 kDa Observed band size: 34 kDa

Applications for CD7 Antibody (307) - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

4-6 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD7

CD7, also known as gp40, is a 40 kD type I transmembrane glycoprotein member of the immunoglobulin superfamily. It is found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion.

Alternate Names

CD7, LEU-9, TP41

Gene Symbol

CD7

Additional CD7 Products

Product Documents for CD7 Antibody (307) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD7 Antibody (307) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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