CD74 Antibody (SPM523)

Novus Biologicals | Catalog # NBP2-32947

Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM523
View all CD74 Primary Antibodies »

Product Specifications

Immunogen

SU-DHL-4 lymphoma cells

Localization

Cell surface and paranuclear globular

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34778)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD74 Antibody (SPM523)

Immunocytochemistry/ Immunofluorescence: CD74 Antibody (SPM523) [NBP2-32947]

Immunocytochemistry/ Immunofluorescence: CD74 Antibody (SPM523) [NBP2-32947]

Immunocytochemistry/Immunofluorescence: CD74 Antibody (SPM523) [NBP2-32947] - Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Antibody (SPM523) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)
Immunohistochemistry-Paraffin: CD74 Antibody (SPM523) [NBP2-32947]

Immunohistochemistry-Paraffin: CD74 Antibody (SPM523) [NBP2-32947]

Immunohistochemistry-Paraffin: CD74 Antibody (SPM523) [NBP2-32947] - Formalin-paraffin human tonsil stained with CD74 Ab (SPM523).
Flow Cytometry: CD74 Antibody (SPM523) [NBP2-32947]

Flow Cytometry: CD74 Antibody (SPM523) [NBP2-32947]

Flow Cytometry: CD74 Antibody (SPM523) [NBP2-32947] - Flow Cytometric Analysis of Human Raji cells using CD74 Antibody (SPM523) followed by Goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).

Applications for CD74 Antibody (SPM523)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD74

CD74, also known as the MHC class II associated invariant chain (Ii), is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is produced in molar excess over MHC class II and some of this is expressed by an unknown pathway on the cell surface independent of, or in association with, MHC class II molecules. The half life of CD74 on the cell surface is only 3 to 4 min after which it is internalized. It has been proposed that cell surface CD74 binds MHC class II molecules which have lost their antigenic peptide leading to internalization and reloading with antigenic peptide. The transport route of class II alpha/beta/Ii complexes is regulated selectively by two forms of CD74 (p33 and p35) which are generated by the use of alternative translation initiation sites. CD74 is expressed primarily by antigen presenting cells such as B lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages and monocytes together, with many epithelial cells. CD74 may exist in different isoforms ranging in size from 33 to 41 kDa, depending on genetic splicing.

Alternate Names

CD74, CLIP, DHLAG, HLADG, Ia-gamma, INVG34

Gene Symbol

CD74

UniProt

P044233 (Human)

Additional CD74 Products

Product Documents for CD74 Antibody (SPM523)

Certificate of Analysis

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Product Specific Notices for CD74 Antibody (SPM523)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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